Rabbit anti-Human NUP153 Polyclonal Antibody | anti-NUP153 antibody

NUP153, NT (NUP153, Nuclear pore complex protein Nup153, 153kD nucleoporin, Nucleoporin Nup153) (PE)

Cat. Num. AAA6327248

• Gene Names: NUP153; N153; HNUP153
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: NUP153; Polyclonal Antibody; NT (NUP153; Nuclear pore complex protein Nup153; 153kD nucleoporin; Nucleoporin Nup153) (PE); Nucleoporin Nup153; anti-NUP153 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NUP153
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NUP153 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: NUP153 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65~94 amino acids from the N-terminal region of human NUP153.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NUP153 Antibody (N-term) in Hela cell line lysates (35ug/lane). NUP153 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(NUP153 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-NUP153 antibody

Antibodies; Nuclear Proteins

References

Olsen, J.V., et.al., Cell 127 (3), 635-648 (2006). Ullman, K.S., et.al., Mol. Biol. Cell 10 (3), 649-664 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 24430146
• NCBI GeneID: 9972
• NCBI Accession #: NP_005115.2
• NCBI GenBank Nucleotide #: NM_005124.2
• UniProt Accession #: P49790; Q5T9I7; Q4LE47; Q7Z743
• Molecular Weight: 153,938 Da
• NCBI Official Full Name: nuclear pore complex protein Nup153
• NCBI Official Synonym Full Names: nucleoporin 153kDa
• NCBI Official Symbol: NUP153
• NCBI Official Synonym Symbols: N153; HNUP153
• NCBI Protein Information: nuclear pore complex protein Nup153; OTTHUMP00000016070; nucleoporin Nup153; 153 kDa nucleoporin; nuclear pore complex protein hnup153
• UniProt Protein Name: Nuclear pore complex protein Nup153
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP153
• UniProt Entry Name: NU153_HUMAN

NCBI Description

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by this gene has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. [provided by RefSeq]

Uniprot Description

NUP153: Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Protein type: Nucleoporin; Nuclear export

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; nuclear pore; nuclear inclusion body

Molecular Function: identical protein binding; protein binding; DNA binding; structural constituent of nuclear pore; zinc ion binding; transporter activity; protein anchor; nucleocytoplasmic transporter activity

Biological Process: nuclear pore complex assembly; entry of virus into host cell; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; negative regulation of RNA export from nucleus; protein transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport