Mouse anti-Human NME1 Monoclonal Antibody | anti-NME1 antibody

NME1 (Nucleoside Diphosphate Kinase A, Granzyme A-activated DNase, GAAD, Metastasis Inhibition Factor nm23, NB, NBS, NDPKA, NDPK-A, NDP Kinase A, NM23, nm23-H1, NM23-H1, Tumor Metastatic Process-associated Protein)

Cat. Num. AAA632615

• Gene Names: NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Synonyms: NME1; Monoclonal Antibody; NME1 (Nucleoside Diphosphate Kinase A; Granzyme A-activated DNase; GAAD; Metastasis Inhibition Factor nm23; NB; NBS; NDPKA; NDPK-A; NDP Kinase A; NM23; nm23-H1; NM23-H1; Tumor Metastatic Process-associated Protein); Anti -NME1 (Nucleoside Diphosphate Kinase A; anti-NME1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10K87
• Specificity: Recognizes human NME1 at ~20kD. Does not crossreact with recombinant human NME2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Form/Format: Supplied as a lyophilized powder from PBS, pH7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
• Concentration: ~0.5mg/mL (after reconstitution) (varies by lot)

• Applicable Applications for anti-NME1 antibody: Western Blot (WB)
• Application Notes: Western Blot: 0.5ug/ml; Optimal dilutions to be determined by the researcher.
• Immunogen: Full length recombiant protein corresponding to Met1-Glu 152 from human NME2, expressed in E.col
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(10ng of rhNM23-H1 (NME1), 10ng of rhNM23-H2 (NME2), and lysates from human A431, HepG2 and K562 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to a PVDF membrane and immunoblotted with 0.5ug/ml of MBS632615)

Related Product Information for anti-NME1 antibody

NME1 (NM23-H1) is a 19-20kD member of the NDK family of enzymes. NME1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NME2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NME1 is 152aa in length, contains one NDP kinase domain (aa5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NME1 shares 89% aa identity with human 17-18kD NME2, and 94% aa identity with mouse NME1. A second H1 isoform named M23-H1B with 25 additional aa at the N-terminus has also been described.

Product Categories/Family for anti-NME1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38045913
• NCBI GeneID: 4830
• NCBI Accession #: NP_937818.1
• NCBI GenBank Nucleotide #: NM_198175.1
• UniProt Accession #: P15531; Q6FGK3; Q86XQ2; Q9UDJ6
• NCBI Official Full Name: nucleoside diphosphate kinase A isoform a
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: NME1
• NCBI Official Synonym Symbols: NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• NCBI Protein Information: nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: NME1
• UniProt Synonym Gene Names: NDPKA; NM23; NDK A; NDP kinase A; GAAD
• UniProt Entry Name: NDKA_HUMAN

NCBI Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.

Protein type: Nucleotide Metabolism - purine; EC 2.7.4.6; Nucleotide Metabolism - pyrimidine; Tumor suppressor; Kinase, nucleoside diphosphate; Kinase, other; Protein kinase, histidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; GTP binding; nucleoside diphosphate kinase activity; magnesium ion binding; deoxyribonuclease activity; ribosomal small subunit binding; ATP binding

Biological Process: GTP biosynthetic process; nervous system development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside diphosphate phosphorylation; endocytosis; DNA catabolic process; regulation of apoptosis; negative regulation of cell proliferation; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; cell differentiation; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; positive regulation of DNA binding

Disease: Neuroblastoma, Susceptibility To