Rabbit anti-Human, Mouse NME1 Polyclonal Antibody | anti-NME1 antibody

NME1, ID (NME1, NDPKA, NM23, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein, nm23-H1)

Cat. Num. AAA646427

• Gene Names: NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NME1; Polyclonal Antibody; ID (NME1; NDPKA; NM23; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein; nm23-H1); Anti -NME1; anti-NME1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NME1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: NME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the Central region of human NME1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A549 cell line and human placenta lysates (35ug/lane) using MBS646427. This demonstrates that MBS646427 detected the NME1 protein (arrow).)

Testing Data

Related Product Information for anti-NME1 antibody

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Product Categories/Family for anti-NME1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38045913
• NCBI GeneID: 4830
• NCBI Accession #: NP_937818.1
• NCBI GenBank Nucleotide #: NM_198175.1
• UniProt Accession #: P15531; Q6FGK3; Q86XQ2; Q9UDJ6
• Molecular Weight: 17,149 Da
• NCBI Official Full Name: nucleoside diphosphate kinase A isoform a
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: NME1
• NCBI Official Synonym Symbols: NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• NCBI Protein Information: nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: NME1
• UniProt Synonym Gene Names: NDPKA; NM23; NDK A; NDP kinase A; GAAD
• UniProt Entry Name: NDKA_HUMAN

NCBI Description

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. Ref.11 Ref.13 Ref.15

Catalytic activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactor: Magnesium.

Enzyme regulation: Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity. Ref.15

Subunit structure: Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with SET and PRUNE. Ref.2 Ref.15 Ref.18

Subcellular location: Cytoplasm. Nucleus. Note: Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA. Ref.16

Tissue specificity: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed. Ref.5 Ref.14 Ref.16

Miscellaneous: The role of this protein in tumor development and progression is uncertain. This protein is found in reduced amount in some tumor cells of high metastatic potential. However, increased NME1 levels correlate with aggressive tumor features in neuroblastoma. May have distinct if not opposite roles in different tumors.

Sequence similarities: Belongs to the NDK family.

Sequence caution: The sequence CAA35621.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.