Mouse anti-Human OCT-2 Monoclonal Antibody | anti-POU2F2 antibody
Oct-2 (Octamer-binding transcription factor 2, Oct-2, OTF-2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2)
Gene Names
POU2F2; OCT2; OTF2; Oct-2
Reactivity
Human
Applications
Immunohistochemistry
Purity
AscitesAscites
Synonyms
OCT-2; Monoclonal Antibody; Oct-2 (Octamer-binding transcription factor 2; Oct-2; OTF-2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2); Anti -Oct-2 (Octamer-binding transcription factor 2; anti-POU2F2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
MRQ-2
Specificity
Recognizes human Oct-2 (Octamer-binding transcription factor 2, Oct-2, OTF-2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2).
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid in PBS, pH7.4, protein base, sodium azide.
Applicable Applications for anti-POU2F2 antibody
Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry (Paraffin, frozen): 1:25-1:100
Dilution: Immunohistochemistry (Paraffin, frozen): 1:25-1:100
Positive Control
Tonsil, lymph node
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-POU2F2 antibody
Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. These are involved in proliferation and differentiation and despite the scarce evidence for Oct-2 expression in T cells, it has been shown that this factor participates in transcriptional regulation during T-cell activation. Oct-2 activity is dependent on phosphorylation and alternatitive splicing, although it seems that the level of its expression can be used as a marker of B-cell lineage and differentiation. The following show high levels of Oct-2 expression: germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells. Various lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, classic Hodgkin lymphoma.
Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines display high levels of expression. Analysis of Oct-2 expression in primary Hodgkin's lymphoma (HLs) and cell lines derived from it showed that the tumor cells analyzed had high levels of expression and activity, suggesting a common B-cell origin for all types of HLs.
Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines display high levels of expression. Analysis of Oct-2 expression in primary Hodgkin's lymphoma (HLs) and cell lines derived from it showed that the tumor cells analyzed had high levels of expression and activity, suggesting a common B-cell origin for all types of HLs.
Product Categories/Family for anti-POU2F2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,209 Da
NCBI Official Full Name
Oct-2
NCBI Official Synonym Full Names
POU class 2 homeobox 2
NCBI Official Symbol
POU2F2
NCBI Official Synonym Symbols
OCT2; OTF2; Oct-2
NCBI Protein Information
POU domain, class 2, transcription factor 2; OTF-2; homeobox protein; OTTHUMP00000231645; OTTHUMP00000231646; OTTHUMP00000231647; OTTHUMP00000231648; OTTHUMP00000231650; octamer-binding protein 2; octamer-binding transcription factor 2; lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
UniProt Protein Name
POU domain, class 2, transcription factor 2
Protein Family
UniProt Gene Name
POU2F2
UniProt Synonym Gene Names
OCT2; OTF2
UniProt Entry Name
PO2F2_HUMAN
NCBI Description
The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Oct2: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. Belongs to the POU transcription factor family. Class- 2 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein domain specific binding; sequence-specific DNA binding; transcription factor activity
Biological Process: immunoglobulin secretion during immune response; transcription from RNA polymerase II promoter; mature B cell differentiation; positive regulation of transcription, DNA-dependent; cell maturation; humoral immune response
Research Articles on POU2F2
Similar Products
Product Notes
The POU2F2 pou2f2 (Catalog #AAA632358) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Oct-2 (Octamer-binding transcription factor 2, Oct-2, OTF-2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OCT-2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry (Paraffin, frozen): 1:25-1:100. Researchers should empirically determine the suitability of the POU2F2 pou2f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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