Rabbit anti-Human MBD2 Polyclonal Antibody | anti-MBD2 antibody

MBD2, ID (MBD2, Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2) (Biotin)

Cat. Num. AAA6319245

• Gene Names: MBD2; DMTase; NY-CO-41
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MBD2; Polyclonal Antibody; ID (MBD2; Methyl-CpG-binding domain protein 2; Demethylase; Methyl-CpG-binding protein MBD2) (Biotin); Methyl-CpG-binding protein MBD2; anti-MBD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MBD2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human MBD2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with MBD2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly.)

Western Blot (WB)

(MBD2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MBD2 antibody detected the MBD2 protein (arrow).)

Related Product Information for anti-MBD2 antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Product Categories/Family for anti-MBD2 antibody

Antibodies; Binding Proteins

References

Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009). McDonough, C.W., et al. Hum. Genet. (2009). In press: Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 7710145
• NCBI GeneID: 8932
• NCBI Accession #: NP_056647.1
• NCBI GenBank Nucleotide #: NM_015832.4
• UniProt Accession #: Q9UBB5; O95242; Q9UIS8
• Molecular Weight: 43,255 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 2 testis-specific isoform
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: MBD2
• NCBI Official Synonym Symbols: DMTase; NY-CO-41
• NCBI Protein Information: methyl-CpG-binding domain protein 2; demethylase
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD2
• UniProt Synonym Gene Names: DMTase
• UniProt Entry Name: MBD2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: nucleoplasm; heterochromatin; protein complex; cytoplasm; nuclear chromatin; histone deacetylase complex; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; nucleosomal DNA binding; siRNA binding; methyl-CpG binding; satellite DNA binding

Biological Process: cellular protein complex assembly; transcription, DNA-dependent; maternal behavior; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of gene expression, epigenetic; regulation of cell proliferation