Western Blot (WB)
(Western Blot (WB) analysis of ZNF76 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264. 7 whole cell lysate Lane3:PC12 whole cell lysate)
Rabbit ZNF76 Polyclonal Antibody | anti-ZNF76 antibody
ZNF76 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ZNF76; Polyclonal Antibody; ZNF76 Polyclonal Antibody; Zinc finger protein 76; Zinc finger protein 523; D6S229E; ZNF523; anti-ZNF76 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ZNF76 polyclonal antibody detects endogenous levels of ZNF76 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
570
Applicable Applications for anti-ZNF76 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human ZNF76.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ZNF76 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264. 7 whole cell lysate Lane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of ZNF76 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264. 7 whole cell lysate Lane3:PC12 whole cell lysate)
Related Product Information for anti-ZNF76 antibody
ZNF76, also known as ZNF523 or Zfp523, is a transcriptional repressor expressed in the testis. It is the human homolog of the Xenopus Staf protein (selenocysteine tRNA gene transcription-activating factor) known to regulate the genes encoding small nuclear RNA and selenocysteine tRNA. ZNF76 localizes to the nucleus and exerts an inhibitory function on p53-mediated transactivation. ZNF76 specifically targets TFIID (TATA-binding protein). The interaction with TFIID occurs through both its N and C termini. The transcriptional repression activity of ZNF76 is predominantly regulated by lysine modifications, acetylation and sumoylation. ZNF76 is sumoylated by PIAS 1 and is acetylated by p300. Acetylation leads to the loss of sumoylation and a weakened TFIID interaction. ZNF76 can be deacetylated by HDAC1. In addition to lysine modifications, ZNF76 activity is also controlled by splice variants. Two isoforms exist due to alternative splicing. These isoforms vary in their ability to interact with TFIID.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 56kDa
NCBI Official Full Name
zinc finger protein 76 isoform 1
UniProt Protein Name
Zinc finger protein 76
Protein Family
UniProt Gene Name
ZNF76
UniProt Synonym Gene Names
D6S229E; ZNF523
UniProt Entry Name
ZNF76_HUMAN
Uniprot Description
ZNF76: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 6p21.31
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription from RNA polymerase III promoter
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Product Notes
The ZNF76 znf76 (Catalog #AAA3007183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF76 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF76 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ZNF76 znf76 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF76, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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