Structure
(Typical Structure of an Antibody)
IgG1 Murine Negative Control Isotype Control
IgG1 Murine Negative Control (For rat tissue) (Azide Free)
Reactivity
Human, Rat
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
IgG1 Murine Negative Control; IgG1 Murine Negative Control (For rat tissue) (Azide Free); IgG1 Murine Negative Control isotype control
Host
Tissue culture supernatant
Reactivity
Human, Rat
Isotype
IgG1
Clone Number
3H1421
Specificity
Recognizes human CD45RA antigen. Note: Use with rat tissues only.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS. No preservatives added.
Applicable Applications for IgG1 Murine Negative Control isotype control
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry used as a matched isotype negative control reagent to asses the level of non-specific binding of mouse IgG1 monoclonal antibodies to rat cells.
Dilution: Flow Cytometry: 10ul of the optimal working dilution to label 10e6 cells in 100ul.. Recommend adjusting the concentration of this reagent to the same as that of the test reagent.
Optimal working dilutions to be determined by researcher.
Dilution: Flow Cytometry: 10ul of the optimal working dilution to label 10e6 cells in 100ul.. Recommend adjusting the concentration of this reagent to the same as that of the test reagent.
Optimal working dilutions to be determined by researcher.
Immunogen
Human T lymphocytes.
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Structure
(Typical Structure of an Antibody)
Structure
(Typical Structure of an Antibody)
Product Categories/Family for IgG1 Murine Negative Control isotype control
Similar Products
Product Notes
The IgG1 Murine Negative Control (Catalog #AAA655701) is an Isotype Control produced from Tissue culture supernatant and is intended for research purposes only. The product is available for immediate purchase. The IgG1 Murine Negative Control (For rat tissue) (Azide Free) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG1 Murine Negative Control can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry used as a matched isotype negative control reagent to asses the level of non-specific binding of mouse IgG1 monoclonal antibodies to rat cells. Dilution: Flow Cytometry: 10ul of the optimal working dilution to label 10e6 cells in 100ul. Recommend adjusting the concentration of this reagent to the same as that of the test reagent. Optimal working dilutions to be determined by researcher. Researchers should empirically determine the suitability of the IgG1 Murine Negative Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG1 Murine Negative Control, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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