Mouse STAT 5 Monoclonal Antibody | anti-Stat92E antibody

STAT 5, phosphorylated (Signal Transducer and Activator of Transcription 5)

Cat. Num. AAA631781

• Gene Names: Stat92E; CG4257; d-STAT; D-stat; D-Stat; D-STAT; D-stat/stat92E; DmelCG4257; DmSTAT; DRODSRC; DSRC; dstat; dSTAT; Dstat; DSTAT; Dstat 92E; dSTAT92E; Dstat92E; dSTAT92E/marelle; l(3)06346; l(3)j6C8; mrl; mrL; mrl stat92E; pSTAT; SD-stat; stat; Stat; STAT; STAT 92E
• Reactivity: Bovine, Human, Rat, Sheep
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: STAT 5; Monoclonal Antibody; phosphorylated (Signal Transducer and Activator of Transcription 5); Anti -STAT 5; anti-Stat92E antibody

• Host: Mouse
• Reactivity: Bovine, Human, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6A188
• Specificity: Recognizes the tyrosine phosphorylated form of STAT5a and STAT5b proteins. It does not cross-react appreciably with the corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine containing proteins. Species sequence homology: human, bovine, sheep, and rat STAT5 proteins.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.4, containing 0.05% sodium azide.

• Applicable Applications for anti-Stat92E antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1-3ug/ml; ELISA: 0.1-1ug/ml
• Immunogen: A synthetic tyrosine phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of murine STAT5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Stat92E antibody

STATs (signal transducers and activators of transcription) were originally identified as DNA-binding proteins essential to interferon IFNa- and IFNg-regulated gene expression. Since then, several additional mammalian STATs, a Drosophila STAT (D-STAT or Marelle), and a STAT-like protein from Dictyostelium discoideum have been recognized. In unstimulated cells STAT proteins exist largely as latent cytoplasmic transcription factors. Treatment of cells with cytokines or certain growth factors results in the tyrosine phosphorylation (via Janus kinases for cytokines) of specific STATs causing them to form homodimers or heterodimers that enter the nucleus. In the nucleus, STAT dimers bind DNA and activate transcription of distinct target genes. Although several different STATs may be activated in response to a given ligand, there is often a striking specificity in the pattern of STAT activation. Mammalian STAT proteins share several conserved structural and functional domains as illustrated below: STAT5 (mammary growth factor) was originally isolated as a tyrosine phosphorylated DNA binding protein from prolactin stimulated mammary tissue and IL-3 stimulated myeloid cells. Subsequently, two highly homologous (~96%) genes, Stat5a and Stat5b, were identified in mouse.7-8 Both STATs are expressed in most tissues of virgin and lactating mice although differential accumulation of STAT5a and STAT5b mRNAs occurs in muscle and mammary tissues. STAT5 activation has been observed in response to IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GM-CSF, TPO, EPO, GH, and prolactin (PRL). STAT5a and STAT5b proteins recognize the GAS (gamma interferon activating sequence) site TTCNNNGAA with the preferred DNA-binding consensus site deduced as TTCC(A>T)GGAA. Western blot analysis of the full-length STAT5a protein, isolated from a variety of cultured cells, indicates that the protein migrates with a molecular weight of ~95kD. C-terminal truncated forms of the STAT5a protein have also been reported.

Product Categories/Family for anti-Stat92E antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 442620068
• NCBI GeneID: 42428
• NCBI Accession #: NP_001262762.1
• NCBI GenBank Nucleotide #: NM_001275833.1
• UniProt Accession #: Q24151; Q0KI43; Q24181; Q59DV7; Q5BIB0; Q8IN54; Q8SX55; Q9VDL8
• Molecular Weight: 86,415 Da
• NCBI Official Full Name: Signal-transducer and activator of transcription protein at 92E, isoform M
• NCBI Official Synonym Full Names: Signal-transducer and activator of transcription protein at 92E
• NCBI Official Symbol: Stat92E
• NCBI Official Synonym Symbols: CG4257; d-STAT; D-stat; D-Stat; D-STAT; D-stat/stat92E; DmelCG4257; DmSTAT; DRODSRC; DSRC; dstat; dSTAT; Dstat; DSTAT; Dstat 92E; dSTAT92E; Dstat92E; dSTAT92E/marelle; l(3)06346; l(3)j6C8; mrl; mrL; mrl stat92E; pSTAT; SD-stat; stat; Stat; STAT; STAT 92E;
• NCBI Protein Information: CG4257 gene product from transcript CG4257-RH; CG4257-PB; CG4257-PC; CG4257-PE; CG4257-PF; CG4257-PG; CG4257-PH; CG4257-PI; CG4257-PJ; CG4257-PK; CG4257-PL; CG4257-PM; Signal-transducer and activator of transcription protein; Signal-transducer and activator of transcription protein at 92E; Stat92E-PB; Stat92E-PC; Stat92E-PE; Stat92E-PF; Stat92E-PG; Stat92E-PH; Stat92E-PI; Stat92E-PJ; Stat92E-PK; Stat92E-PL; Stat92E-PM; marelle; signal transducer and activator of transcription; signal transducers and activator of transcription
• UniProt Protein Name: Signal transducer and transcription activator
• UniProt Gene Name: Stat92E
• UniProt Synonym Gene Names: MARE; mrL; Stat; d-STAT
• UniProt Entry Name: STAT_DROME

Uniprot Description

STAT5A: Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression. The Janus kinase-STAT pathway is connected to drosophila early development

Protein type: DNA-binding; Motility/polarity/chemotaxis; Oncoprotein; Transcription factor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; signal transducer activity; DNA binding; transcription factor activity

Biological Process: oogenesis; negative regulation of Wnt receptor signaling pathway; lymph gland hemopoiesis; somatic stem cell maintenance; primary sex determination; stem cell division; compound eye development; defense response; negative regulation of antimicrobial humoral response; signal transduction; segment specification; regulation of JAK-STAT cascade; regulation of transcription, DNA-dependent; hemocyte proliferation; positive regulation of cell proliferation; somatic stem cell division; hemopoiesis; epithelial cell fate determination, open tracheal system; nervous system development; germ-line stem cell division; imaginal disc-derived wing morphogenesis; larval somatic muscle development; stem cell maintenance; oocyte localization during germarium-derived egg chamber formation; germ-line stem cell maintenance; ovarian follicle cell stalk formation; JAK-STAT cascade; border follicle cell migration; humoral immune response; sex determination; long-term memory; open tracheal system development; cell dedifferentiation; lamellocyte differentiation; hindgut morphogenesis; positive regulation of defense response to virus by host; eye-antennal disc morphogenesis; blastoderm segmentation