Rabbit anti-Human LRRC33 Polyclonal Antibody | anti-LRRC33 antibody

LRRC33, CT (LRRC33, Leucine-rich repeat-containing protein 33) (Azide free) (HRP)

Cat. Num. AAA6317069

• Gene Names: NRROS; GARPL1; LRRC33; UNQ3030
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LRRC33; Polyclonal Antibody; CT (LRRC33; Leucine-rich repeat-containing protein 33) (Azide free) (HRP); Leucine-rich repeat-containing protein 33; anti-LRRC33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-LRRC33 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LRRC33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-648 amino acids from the C-terminal region of human LRRC33.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LRRC33 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LRRC33 antibody detected the LRRC33 protein (arrow).)

Immunohistochemistry (IHC)

(LRRC33 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph node followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LRRC33 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-LRRC33 antibody

Antibodies; Leucine-rich Proteins

References

Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 38348406
• NCBI GeneID: 375387
• NCBI Accession #: NP_940967.1
• NCBI GenBank Nucleotide #: NM_198565.1
• UniProt Accession #: Q86YC3
• Molecular Weight: 76,366 Da
• NCBI Official Full Name: leucine-rich repeat-containing protein 33
• NCBI Official Synonym Full Names: negative regulator of reactive oxygen species
• NCBI Official Symbol: NRROS
• NCBI Official Synonym Symbols: GARPL1; LRRC33; UNQ3030
• NCBI Protein Information: leucine-rich repeat-containing protein 33; ELLP3030; leucine rich repeat containing 33
• UniProt Protein Name: Leucine-rich repeat-containing protein 33
• UniProt Gene Name: NRROS
• UniProt Synonym Gene Names: LRRC33
• UniProt Entry Name: LRC33_HUMAN

Uniprot Description

NRROS: May play a critical role in desensitizing TLR signaling through inhibition of Toll-like receptor-mediated NF-kappa-B activation and cytokine production. Interacts (via LRR repeats) with TLR2, TLR3, TLR4, TLR9 and probably other Toll-like receptors

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; plasma membrane

Biological Process: superoxide metabolic process; innate immune response; immune response; inflammatory response