Rabbit anti-Human KRIT1 Polyclonal Antibody | anti-KRIT1 antibody

KRIT1, NT (KRIT1, CCM1, Krev interaction trapped protein 1, Cerebral cavernous malformations 1 protein) (APC)

Cat. Num. AAA6314514

• Gene Names: KRIT1; CAM; CCM1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRIT1; Polyclonal Antibody; NT (KRIT1; CCM1; Krev interaction trapped protein 1; Cerebral cavernous malformations 1 protein) (APC); Cerebral cavernous malformations 1 protein; anti-KRIT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KRIT1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KRIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human KRIT1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KRIT1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the KRIT1 antibody detected the KRIT1 protein (arrow).)

Related Product Information for anti-KRIT1 antibody

This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-KRIT1 antibody

Antibodies; Proteins

References

Reddy, S., et al. Graefes Arch. Clin. Exp. Ophthalmol. 248(9):1359-1361(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Stockton, R.A., et al. J. Exp. Med. 207(4):881-896(2010). Petersen, T.A., et al. AJNR Am J Neuroradiol 31(2):377-382(2010). Lee, Y.W., et al. Ann. Clin. Lab. Sci. 40(3):290-294(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 61742817
• NCBI GeneID: 889
• NCBI Accession #: NP_004903.2
• NCBI GenBank Nucleotide #: NM_004912.3
• UniProt Accession #: O00522; O43894; Q506L6; Q6U276; Q75N19; Q9H180; Q9H264; Q9HAX5; A6NNU0
• Molecular Weight: 84,348 Da
• NCBI Official Full Name: krev interaction trapped protein 1 isoform 2
• NCBI Official Synonym Full Names: KRIT1, ankyrin repeat containing
• NCBI Official Symbol: KRIT1
• NCBI Official Synonym Symbols: CAM; CCM1
• NCBI Protein Information: krev interaction trapped protein 1; krev interaction trapped 1; ankyrin repeat-containing protein Krit1; cerebral cavernous malformations 1 protein
• UniProt Protein Name: Krev interaction trapped protein 1
• Protein Family: Krev interaction trapped protein
• UniProt Gene Name: KRIT1
• UniProt Synonym Gene Names: CCM1; Krev interaction trapped 1

NCBI Description

This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

KRIT1: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits EKR1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels. Defects in KRIT1 are the cause of cerebral cavernous malformations type 1 (CCM1). Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: extracellular space; intercellular junction; microtubule; plasma membrane

Molecular Function: GTPase regulator activity; microtubule binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein complex binding

Biological Process: cell redox homeostasis; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; small GTPase mediated signal transduction

Disease: Cerebral Cavernous Malformations