Rabbit anti-Human ITK Polyclonal Antibody | anti-ITK antibody

ITK, NT (ITK, EMT, LYK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk) (Biotin)

Cat. Num. AAA6311347

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ITK; Polyclonal Antibody; NT (ITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk) (Biotin); Tyrosine-protein kinase Lyk; anti-ITK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITK.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ITK antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 25-54 in the N-terminal region of human ITK, UniProt Accession #Q08881. Species sequence homology: mouse
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of A549 cell line lysates (35ug/lane) usingMBS6011999. This demonstrates MBS6011999 detected the ITK protein (arrow).)

Related Product Information for anti-ITK antibody

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

Product Categories/Family for anti-ITK antibody

Antibodies; Protein Kinases

References

1. Grasis, J.A., et al. Mol. Cell. Biol. 30(14):3596-3609(2010). 2. Pechloff, K., et al. J. Exp. Med. 207(5):1031-1044(2010). 3. Qi, Q., et al. J. Biol. Chem. 284(43):29882-29892(2009). 4. Kogina, K., et al. Mol. Cells 28(2):125-130(2009). 5. Voss, M., et al. BMC Immunol. 10, 53 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 15718680
• NCBI GeneID: 3702
• NCBI Accession #: NP_005537.3
• NCBI GenBank Nucleotide #: NM_005546.3
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 71,831 Da
• NCBI Official Full Name: tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2 inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]