B-Lymphoid Tyrosine Kinase Recombinant Protein | BLK recombinant protein

Recombinant B-Lymphoid Tyrosine Kinase (BLK)

Cat. Num. AAA2031774

• Gene Names: BLK; MODY11
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: B-Lymphoid Tyrosine Kinase; Recombinant B-Lymphoid Tyrosine Kinase (BLK); BLK recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; LRLVRKLGSGQFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLYAVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAYIERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIKWTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLERGYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFY
• Sequence Length: 505

• Applicable Applications for BLK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.7KDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Leu241~Tyr494
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21595367
• NCBI GeneID: 640
• Molecular Weight: 57,706 Da
• NCBI Official Full Name: B lymphoid tyrosine kinase
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK
• UniProt Entry Name: BLK_HUMAN

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; positive regulation of insulin secretion; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation

Disease: Maturity-onset Diabetes Of The Young, Type 11