Rabbit anti-Human IMP2L Polyclonal Antibody | anti-IMP2L antibody

IMP2L, ID (IMMP2L, Mitochondrial inner membrane protease subunit 2, IMP2-like protein) (PE)

Cat. Num. AAA6310398

• Gene Names: IMMP2L; IMP2; IMP2-LIKE; IMMP2L-IT1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IMP2L; Polyclonal Antibody; ID (IMMP2L; Mitochondrial inner membrane protease subunit 2; IMP2-like protein) (PE); IMMP2L; mitochondrial inner membrane protease subunit 2; IMP2-like protein; anti-IMP2L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-IMP2L antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IMP2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~117 amino acids from the Central region of human IMP2L.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IMP2L Antibody (Center) in Jurkat cell line lysates (35ug/lane). IMP2L (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(IMP2L Antibody (Center) IHC analysis in formalin fixed and paraffin embedded rat lung tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IMP2L Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IMP2L Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-IMP2L antibody

IMP2L complex generates mature, active proteins in the mitochondrial intermembrane space by proteolytically removing the mitochondrial targeting presequence of nuclear-encoded proteins. IMP1 (IMMP1L; MIM 612323) and IMP2 are the catalytic subunits of the IMP complex.

Product Categories/Family for anti-IMP2L antibody

Antibodies; Mitochondrial Proteins

References

Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009). Trynka, G., et al. Gut 58(8):1078-1083(2009). de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 347446661
• NCBI GeneID: 83943
• NCBI Accession #: NP_001231535.1
• NCBI GenBank Nucleotide #: NM_001244606.1
• UniProt Accession #: Q96T52; Q75MF1; Q75MN9; Q75MP0; Q75MS5; Q75MS8; Q96HJ2
• Molecular Weight: 12,259 Da
• NCBI Official Full Name: mitochondrial inner membrane protease subunit 2
• NCBI Official Synonym Full Names: inner mitochondrial membrane peptidase subunit 2
• NCBI Official Symbol: IMMP2L
• NCBI Official Synonym Symbols: IMP2; IMP2-LIKE; IMMP2L-IT1
• NCBI Protein Information: mitochondrial inner membrane protease subunit 2
• UniProt Protein Name: Mitochondrial inner membrane protease subunit 2
• UniProt Gene Name: IMMP2L
• UniProt Entry Name: IMP2L_HUMAN

Uniprot Description

IMMP2L: Catalyzes the removal of transit peptides required for the targeting of proteins from the mitochondrial matrix, across the inner membrane, into the inter-membrane space. Known to process the nuclear encoded protein DIABLO. Defects in IMMP2L may be a cause of Gilles de la Tourette syndrome (GTS). GTS is a neurologic disorder manifested particularly by motor and vocal tics and associated with behavioral abnormalities. Belongs to the peptidase S26 family. IMP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Membrane protein, integral; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 7q31

Molecular Function: peptidase activity; serine-type endopeptidase activity

Biological Process: mitochondrial protein processing; mitochondrial respiratory chain complex assembly; signal peptide processing