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Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6001086. This demonstrates that MBS6001086 detected the IL1RL2 protein (arrow).)

Rabbit anti-Human IL1RL2 Polyclonal Antibody | anti-IL1RL2 antibody

IL1RL2, ID (IL1RL2, IL1RRP2, Interleukin-1 receptor-like 2, Interleukin-1 receptor-related protein 2) (Biotin)

Gene Names
IL1RL2; IL-36R; IL1RRP2; IL-1Rrp2; IL1R-rp2
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
IL1RL2; Polyclonal Antibody; ID (IL1RL2; IL1RRP2; Interleukin-1 receptor-like 2; Interleukin-1 receptor-related protein 2) (Biotin); Interleukin-1 receptor-related protein 2; anti-IL1RL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IL1RL2.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-IL1RL2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 257-286 in the central region of human IL1RL2, Swiss/UniProt Accession: Q9HB29. Cellular Localization: Membrane; single-pass type 1 membrane protein.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6001086. This demonstrates that MBS6001086 detected the IL1RL2 protein (arrow).)

Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6001086. This demonstrates that MBS6001086 detected the IL1RL2 protein (arrow).)

Testing Data

(All lanes: MBS6001086 (1:8000) Lane 1: HL-60 whole cell lysate Lane 2: U-87 MG whole cell lysate Lane 3: K562 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG at 1:10,000. Predicted band size: 65kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data (All lanes: MBS6001086 (1:8000) Lane 1: HL-60 whole cell lysate Lane 2: U-87 MG whole cell lysate Lane 3: K562 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit IgG at 1:10,000. Predicted band size: 65kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(Overlay histogram showing K562 cells stained with MBS6001086 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6001086 (1:25) for 60min at 37 degree C. The secondary antibody Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1:200) for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Testing Data (Overlay histogram showing K562 cells stained with MBS6001086 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6001086 (1:25) for 60min at 37 degree C. The secondary antibody Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1:200) for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Related Product Information for anti-IL1RL2 antibody
The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq].
Product Categories/Family for anti-IL1RL2 antibody
References
1. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 2. Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). 3. Nakki, A., et al. BMC Med. Genet. 11 (1), 50 (2010). 4. Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). 5. Solovieva, S., et al. J. Rheumatol. 36(9):1977-1986(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,658 Da
NCBI Official Full Name
interleukin-1 receptor-like 2
NCBI Official Synonym Full Names
interleukin 1 receptor-like 2
NCBI Official Symbol
IL1RL2
NCBI Official Synonym Symbols
IL-36R; IL1RRP2; IL-1Rrp2; IL1R-rp2
NCBI Protein Information
interleukin-1 receptor-like 2; IL-36 receptor; IL-1 receptor-related protein 2; interleukin-1 receptor-related protein 2
UniProt Protein Name
Interleukin-1 receptor-like 2
Protein Family
UniProt Gene Name
IL1RL2
UniProt Synonym Gene Names
IL1RRP2; IL-36R; IL-1Rrp2; IL1R-rp2
UniProt Entry Name
ILRL2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1RL2: Receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. Belongs to the interleukin-1 receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: integral to plasma membrane

Molecular Function: interleukin-1, Type I, activating receptor activity; interleukin-1 receptor activity

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; regulation of inflammatory response; innate immune response; positive regulation of interleukin-6 production; cellular defense response; inflammatory response; signal transduction

Research Articles on IL1RL2

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Product Notes

The IL1RL2 il1rl2 (Catalog #AAA6310060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL1RL2, ID (IL1RL2, IL1RRP2, Interleukin-1 receptor-like 2, Interleukin-1 receptor-related protein 2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL1RL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IL1RL2 il1rl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1RL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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