Rabbit anti-Mouse Ikbke Polyclonal Antibody | anti-Ikbke antibody

Ikbke, ID (Ikbke, Ikke, Ikki, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase) (FITC)

Cat. Num. AAA6309643

• Gene Names: Ikbke; Ikki; IKK-i; AW558201; IKKepsilon
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ikbke; Polyclonal Antibody; ID (Ikbke; Ikke; Ikki; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; Inducible I kappa-B kinase) (FITC); Inducible I kappa-B kinase; anti-Ikbke antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Ikbke antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Ikbke antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 469-498 amino acids from the Central region of Mouse Ikbke.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Ikbke Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Ikbke antibody detected the Ikbke protein (arrow).)

Related Product Information for anti-Ikbke antibody

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death (By similarity).

Product Categories/Family for anti-Ikbke antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 31981053
• NCBI GeneID: 56489
• NCBI Accession #: NP_062751.2
• NCBI GenBank Nucleotide #: NM_019777.3
• UniProt Accession #: Q9R0T8; Q8C2I3
• Molecular Weight: 80,953 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit epsilon
• NCBI Official Synonym Full Names: inhibitor of kappaB kinase epsilon
• NCBI Official Symbol: Ikbke
• NCBI Official Synonym Symbols: Ikki; IKK-i; AW558201; IKKepsilon
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit epsilon
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: Ikbke
• UniProt Synonym Gene Names: Ikke; Ikki; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i
• UniProt Entry Name: IKKE_MOUSE

Uniprot Description

IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.

Protein type: Kinase, protein; EC 2.7.11.10; Protein kinase, Ser/Thr (non-receptor); Inhibitor; Protein kinase, Other; Other group; IKK family

Cellular Component: nucleoplasm; PML body; mitochondrial membrane; cytoplasm; nucleus

Molecular Function: transferase activity; NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; protein binding; IkappaB kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; I-kappaB phosphorylation; phosphorylation; response to DNA damage stimulus; protein amino acid phosphorylation