Rabbit Jun B Polyclonal Antibody | anti-JUN antibody

Jun B, phosphorylated (Ser259) (JunB, Jun B Proto-oncogene, Transcription Factor Jun-B)

Cat. Num. AAA630789

• Gene Names: JUN; AP1; AP-1; c-Jun
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Jun B; Polyclonal Antibody; phosphorylated (Ser259) (JunB; Jun B Proto-oncogene; Transcription Factor Jun-B); Anti -Jun B; anti-JUN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Jun B when phosphorylated at serine 259. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-JUN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human JunB around the phosphorylation site of serine 259 (P-V-SP-P-I).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-JUN antibody

The JunB transcription factor, also known as JunB proto-oncogene, JunB oncogene, transcription factor JunB, and transforming protein JunB, is a basic region leucine zipper DNA-binding protein, and a member of the Jun family of proteins, which also includes c-Jun and JunD. Jun proteins can homodimerize or heterodimerize with other Jun family members, or heterodimerize with Fos family members (cFos, FosB, Fra-1, and Fra-2) to form the activator protein 1 (AP-1) transcription factor. The various dimers differ in their ability to transactivate AP-1-dependent genes. Jun B8,9 and JunD1,10 are almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. JunB and C-Jun antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cyclin A is a direct transcriptional target of JunB driving cell proliferation JunB is an important regulator of erythroid differentiation, and is also important for the development of Th2 effector cells, normal Th2 cytokine expression pattern, and a complete Th2- dependent immune response in mice. JunB expression levels are increased in human colorectal adenocarcinoma specimens as compared to normal mucosa.

Product Categories/Family for anti-JUN antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 386839
• NCBI GeneID: 3725
• UniProt Accession #: P05412; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: JUN
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1; p39; jun oncogene; activator protein 1; proto-oncogene c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; v-jun sarcoma virus 17 oncogene homolog; v-jun avian sarcoma virus 17 oncogene homolog
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: Transcription factor
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Jun: the protooncogene c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The Jun Kinases (JNKs) binds to the N-terminal region of c-Jun and phosphorylate it in response to extracellular signals including growth factors, cytokines and stress, regulating its transcriptional activity.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: nucleoplasm; nuclear chromosome; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; transcription coactivator activity; double-stranded DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of monocyte differentiation; circadian rhythm; response to cAMP; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; stress-activated MAPK cascade; microglial cell activation; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; SMAD protein nuclear translocation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; response to radiation; positive regulation of fibroblast proliferation; regulation of transcription factor activity; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of neuron apoptosis; angiogenesis; toll-like receptor 4 signaling pathway; aging; response to drug; release of cytochrome c from mitochondria; monocyte differentiation; leading edge cell differentiation; axon regeneration; MyD88-independent toll-like receptor signaling pathway; learning; liver development; negative regulation of DNA binding; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; membrane depolarization; cellular response to potassium ion starvation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication