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Western Blot (WB) (Western Blot analysis of GYPB (arrow) usingMBS6004892. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GYPB gene.)

Rabbit anti-Human Glycophorin-B Polyclonal Antibody | anti-GYPB antibody

Glycophorin-B, ID (GYPB, GPB,, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, CD235b) (Azide free) (HRP)

Gene Names
GYPB; SS; GPB; MNS; PAS-3; CD235b
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
Glycophorin-B; Polyclonal Antibody; ID (GYPB; GPB; ; PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; CD235b) (Azide free) (HRP); GYPB; CD235b; anti-GYPB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-GYPB antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa22-51 in the central region of human Glycophorin-B, UniProt Accession #P06028; NP_002091.3
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of GYPB (arrow) usingMBS6004892. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GYPB gene.)

Western Blot (WB) (Western Blot analysis of GYPB (arrow) usingMBS6004892. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GYPB gene.)

Immunohistochemistry (IHC)

(GYPB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunohistochemistry (IHC) (GYPB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Related Product Information for anti-GYPB antibody
This protein is a minor sialoglycoprotein in erythrocyte membranes.
Product Categories/Family for anti-GYPB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
6,352 Da
NCBI Official Full Name
glycophorin-B isoform 1
NCBI Official Synonym Full Names
glycophorin B (MNS blood group)
NCBI Official Symbol
GYPB
NCBI Official Synonym Symbols
SS; GPB; MNS; PAS-3; CD235b
NCBI Protein Information
glycophorin-B; SS-active sialoglycoprotein; Ss blood group; sialoglycoprotein delta
UniProt Protein Name
Glycophorin-B
Protein Family
UniProt Gene Name
GYPB
UniProt Synonym Gene Names
GPB

NCBI Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

GYPB: This protein is a minor sialoglycoprotein in erythrocyte membranes. Belongs to the glycophorin-A family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: leukocyte migration

Disease: Malaria, Susceptibility To

Research Articles on GYPB

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Product Notes

The GYPB gypb (Catalog #AAA6305189) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glycophorin-B, ID (GYPB, GPB,, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, CD235b) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glycophorin-B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GYPB gypb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycophorin-B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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