Rabbit anti-Human, Mouse GHSR Polyclonal Antibody | anti-GHSR antibody

GHSR, CT (GHSR, Growth hormone secretagogue receptor type 1, GH-releasing peptide receptor, Ghrelin receptor) (MaxLight 650)

Cat. Num. AAA6302212

• Gene Names: GHSR; GHDP
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GHSR; Polyclonal Antibody; CT (GHSR; Growth hormone secretagogue receptor type 1; GH-releasing peptide receptor; Ghrelin receptor) (MaxLight 650); Ghrelin receptor; anti-GHSR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GHSR. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-GHSR antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 326-357 in the C-terminal region of human GHSR. Species sequence homology: porcine and rabbit
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292 cell line and mouse bladder tissue lysates (35ug/lane) usingMBS6005342. This demonstrates that MBS6005342 detected the GHSR protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue using MBS6005342 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005342 for immunohistochemistry.)

Product Categories/Family for anti-GHSR antibody

Antibodies; Receptors

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Landgren, S., et al. Alcohol. Clin. Exp. Res. 34(9):1519-1524(2010). 3. Aghajanova, L., et al. Reprod Sci 17(9):823-832(2010). 4. Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010). 5. Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 38455410
• NCBI GeneID: 2693
• NCBI Accession #: NP_940799
• NCBI GenBank Nucleotide #: NM_198407
• UniProt Accession #: Q92847
• Molecular Weight: 40kDa
• NCBI Official Full Name: growth hormone secretagogue receptor type 1 isoform 1a
• NCBI Official Synonym Full Names: growth hormone secretagogue receptor
• NCBI Official Symbol: GHSR
• NCBI Official Synonym Symbols: GHDP
• NCBI Protein Information: growth hormone secretagogue receptor type 1
• UniProt Protein Name: Growth hormone secretagogue receptor type 1
• Protein Family: Growth hormone secretagogue receptor
• UniProt Gene Name: GHSR
• UniProt Synonym Gene Names: GHS-R; GHRP
• UniProt Entry Name: GHSR_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations in this gene are associated with autosomal idiopathic short stature.[provided by RefSeq, Apr 2010]

Uniprot Description

GHSR: Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine). Defects in GHSR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: neuron projection; cell surface; plasma membrane; integral to membrane; lipid raft

Molecular Function: G-protein coupled receptor activity; growth hormone secretagogue receptor activity; peptide hormone binding; growth hormone-releasing hormone receptor activity

Biological Process: response to food; positive regulation of insulin-like growth factor receptor signaling pathway; hormone-mediated signaling; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; adult feeding behavior; positive regulation of multicellular organism growth; decidualization; negative regulation of interleukin-1 beta production; negative regulation of insulin secretion; growth hormone secretion; G-protein coupled receptor protein signaling pathway; positive regulation of fatty acid metabolic process; regulation of synaptogenesis; cellular response to insulin stimulus; negative regulation of inflammatory response; actin polymerization and/or depolymerization; positive regulation of appetite; regulation of hindgut contraction; negative regulation of tumor necrosis factor biosynthetic process

Disease: Short Stature, Idiopathic, Autosomal