Rabbit anti-Human, Mouse GGA1 Polyclonal Antibody | anti-GGA1 antibody
GGA1, NT (GGA1, ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1) (FITC)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(The anti-GGA1 Pab is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).)
Immunostaining (IS)
(A, N2a cells cotransfected with APP770-V5 and GGA1-myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695-GFP and GGA1-myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27;26(39):9913-9922))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GGA1: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: nucleoplasm; Golgi apparatus; clathrin adaptor complex; intracellular membrane-bound organelle; membrane; endosome membrane
Molecular Function: protein binding
Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process