Rabbit anti-Human FREQ Polyclonal Antibody | anti-FREQ antibody

FREQ, NT (NCS1, FLUP, FREQ, Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, Frequenin-like ubiquitous protein) (Biotin)

Cat. Num. AAA6300094

• Gene Names: NCS1; FLUP; FREQ
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FREQ; Polyclonal Antibody; NT (NCS1; FLUP; Neuronal calcium sensor 1; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein) (Biotin); NCS1; Frequenin-like ubiquitous protein; anti-FREQ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FREQ antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FREQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FREQ.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FREQ Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FREQ antibody detected the FREQ protein (arrow).)

Immunohistochemistry (IHC)

(FREQ Antibody (N-term) (AP14572a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FREQ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FREQ antibody

This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-FREQ antibody

Antibodies; Neuroscience

References

Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Handley, M.T., et al. PLoS ONE 5 (5), E10534 (2010): Torres, K.C., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 33(2):229-234(2009). Szafranski, K., et al. Genome Biol. 8 (8), R154 (2007): Kapp-Barnea, Y., et al. Mol. Biol. Cell 17(9):4130-4141(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 192447423
• NCBI GeneID: 23413
• NCBI Accession #: NP_001122298.1; NP_055101.2
• NCBI GenBank Nucleotide #: NM_001128826.1
• UniProt Accession #: P62166; P36610; Q9UK26; E9PAY3
• Molecular Weight: 19,728 Da
• NCBI Official Full Name: neuronal calcium sensor 1 isoform 2
• NCBI Official Synonym Full Names: neuronal calcium sensor 1
• NCBI Official Symbol: NCS1
• NCBI Official Synonym Symbols: FLUP; FREQ
• NCBI Protein Information: neuronal calcium sensor 1
• UniProt Protein Name: Neuronal calcium sensor 1
• Protein Family: Frequenin
• UniProt Gene Name: NCS1
• UniProt Synonym Gene Names: FLUP; FREQ; NCS-1
• UniProt Entry Name: NCS1_HUMAN

Uniprot Description

FREQ: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with IL1RAPL1. Belongs to the recoverin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoplasm; intracellular membrane-bound organelle; plasma membrane

Molecular Function: calcium ion binding; protein binding; voltage-gated calcium channel activity