Rabbit anti-Human ESSPL Polyclonal Antibody | anti-ESSPL antibody

ESSPL, ID (PRSS48, ESSPL, Serine protease 48, Epidermis-specific serine protease-like protein) (Biotin)

Cat. Num. AAA6296879

• Gene Names: PRSS48; ESSPL
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ESSPL; Polyclonal Antibody; ID (PRSS48; Serine protease 48; Epidermis-specific serine protease-like protein) (Biotin); PRSS48; Epidermis-specific serine protease-like protein; anti-ESSPL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ESSPL antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ESSPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the Central region of human ESSPL.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ESSPL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ESSPL antibody detected the ESSPL protein (arrow).)

Flow Cytometry (FC/FACS)

(ESSPL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ESSPL antibody

ESSPL belongs to the peptidase S1 family, however, the specific function is not yet known. There are three different isoforms.

Product Categories/Family for anti-ESSPL antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 148806900
• NCBI GeneID: 345062
• NCBI Accession #: NP_899231.2
• NCBI GenBank Nucleotide #: NM_183375.2
• UniProt Accession #: Q7RTY5; Q08E82; Q0VAD4
• Molecular Weight: 20,230 Da
• NCBI Official Full Name: serine protease 48
• NCBI Official Synonym Full Names: serine protease 48
• NCBI Official Symbol: PRSS48
• NCBI Official Synonym Symbols: ESSPL
• NCBI Protein Information: serine protease 48
• UniProt Protein Name: Serine protease 48
• UniProt Gene Name: PRSS48
• UniProt Synonym Gene Names: ESSPL
• UniProt Entry Name: PRS48_HUMAN

Uniprot Description

ESSPL: Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: proteolysis