Rabbit anti-Human EphA1 Polyclonal Antibody | anti-EphA1 antibody

EphA1, NT (EPHA1, EPH, EPHT, EPHT1, Ephrin type-A receptor 1, EPH tyrosine kinase, EPH tyrosine kinase 1, Erythropoietin-producing hepatoma receptor, Tyrosine-protein kinase receptor EPH) (APC)

Cat. Num. AAA6295964

• Gene Names: EPHA1; EPH; EPHT; EPHT1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: EphA1; Polyclonal Antibody; NT (EPHA1; EPH; EPHT; EPHT1; Ephrin type-A receptor 1; EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH) (APC); EPHA1; Tyrosine-protein kinase receptor EPH; anti-EphA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphA1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EphA1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 29-60 in the N-terminal region of human EphA1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HeLa cell lysate usingMBS643252. EphA1 (arrow) was detected usingMBS643252. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS643252, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS643252 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells using MBS643252 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EphA1 antibody

Antibodies; Protein Kinases

References

1. Hillier, L.W., et al., Nature 424(6945):157-164 (2003). 2. Owshalimpur, D., et al., Mol. Cell. Probes 13(3):169-173 (1999). 3. Hirai, H., et al., Science 238(4834):1717-1720 (1987).

NCBI and Uniprot Product Information

• NCBI GI #: 221316650
• NCBI GeneID: 2041
• NCBI Accession #: NP_005223.4
• NCBI GenBank Nucleotide #: NM_005232.4
• UniProt Accession #: P21709; Q15405; A1L3V3
• Molecular Weight: 108,127 Da
• NCBI Official Full Name: ephrin type-A receptor 1
• NCBI Official Synonym Full Names: EPH receptor A1
• NCBI Official Symbol: EPHA1
• NCBI Official Synonym Symbols: EPH; EPHT; EPHT1
• NCBI Protein Information: ephrin type-A receptor 1; hEpha1; oncogene EPH; eph tyrosine kinase 1; soluble EPHA1 variant 1; soluble EPHA1 variant 2; tyrosine-protein kinase receptor EPH; erythropoietin-producing hepatoma receptor; erythropoietin-producing hepatoma amplified sequence
• UniProt Protein Name: Ephrin type-A receptor 1
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA1
• UniProt Synonym Gene Names: EPH; EPHT; EPHT1; hEpha1
• UniProt Entry Name: EPHA1_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: transmembrane-ephrin receptor activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of angiogenesis; peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; somatic stem cell maintenance; protein amino acid autophosphorylation; positive regulation of cell proliferation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of protein kinase activity; angiogenesis; positive regulation of cell-matrix adhesion; negative regulation of cell migration; positive regulation of cell migration