Rabbit anti-Human DUS2L Polyclonal Antibody | anti-DUS2L antibody

DUS2L, CT (DUS2L, DUS2, tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, Up-regulated in lung cancer protein 8, tRNA-dihydrouridine synthase 2-like) (FITC)

Cat. Num. AAA6293744

• Gene Names: DUS2; SMM1; DUS2L; URLC8
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUS2L; Polyclonal Antibody; CT (DUS2L; DUS2; tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; Up-regulated in lung cancer protein 8; tRNA-dihydrouridine synthase 2-like) (FITC); tRNA-dihydrouridine synthase 2-like; anti-DUS2L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 493

• Applicable Applications for anti-DUS2L antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUS2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-397 amino acids from the C-terminal region of human DUS2L.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DUS2L Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DUS2L antibody detected the DUS2L protein (arrow).)

Related Product Information for anti-DUS2L antibody

Dihydrouridine synthase catalyzes reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al., 2005 [PubMed 15994936]).

Product Categories/Family for anti-DUS2L antibody

Antibodies; Cancer Markers

References

Mittelstadt, M., et al. Nucleic Acids Res. 36(3):998-1008(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Kato, T., et al. Cancer Res. 65(13):5638-5646(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 8923374
• NCBI GeneID: 54920
• NCBI Accession #: NP_060273.1
• NCBI GenBank Nucleotide #: NP_060273.1
• UniProt Accession #: Q9NX74
• NCBI Official Full Name: tRNA-dihydrouridine(20) synthase
• NCBI Official Synonym Full Names: dihydrouridine synthase 2
• NCBI Official Symbol: DUS2
• NCBI Official Synonym Symbols: SMM1; DUS2L; URLC8
• NCBI Protein Information: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Protein Name: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Gene Name: DUS2L
• UniProt Synonym Gene Names: DUS2; URLC8; hDUS2
• UniProt Entry Name: DUS2L_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

DUS2L: Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus2 subfamily.

Protein type: RNA-binding; Oxidoreductase; EC 1.3.1.-; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm

Molecular Function: protein binding; FAD binding; protein kinase inhibitor activity; double-stranded RNA binding; tRNA dihydrouridine synthase activity

Biological Process: negative regulation of protein kinase activity