Rabbit anti-Human DUS2L Polyclonal Antibody | anti-DUS2L antibody

DUS2L, CT (DUS2L, DUS2, tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, Up-regulated in lung cancer protein 8, tRNA-dihydrouridine synthase 2-like) (Azide free) (HRP)

Cat. Num. AAA6293745

• Gene Names: DUS2; SMM1; DUS2L; URLC8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUS2L; Polyclonal Antibody; CT (DUS2L; DUS2; tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; Up-regulated in lung cancer protein 8; tRNA-dihydrouridine synthase 2-like) (Azide free) (HRP); tRNA-dihydrouridine synthase 2-like; anti-DUS2L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 493

• Applicable Applications for anti-DUS2L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUS2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-397 amino acids from the C-terminal region of human DUS2L.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DUS2L Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the DUS2L antibody detected the DUS2L protein (arrow).)

Related Product Information for anti-DUS2L antibody

Dihydrouridine synthase catalyzes reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al., 2005 [PubMed 15994936]).

Product Categories/Family for anti-DUS2L antibody

Antibodies; Cancer Markers

References

Mittelstadt, M., et al. Nucleic Acids Res. 36(3):998-1008(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Kato, T., et al. Cancer Res. 65(13):5638-5646(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 8923374
• NCBI GeneID: 54920
• NCBI Accession #: NP_060273.1
• NCBI GenBank Nucleotide #: NP_060273.1
• UniProt Accession #: Q9NX74
• NCBI Official Full Name: tRNA-dihydrouridine(20) synthase
• NCBI Official Synonym Full Names: dihydrouridine synthase 2
• NCBI Official Symbol: DUS2
• NCBI Official Synonym Symbols: SMM1; DUS2L; URLC8
• NCBI Protein Information: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Protein Name: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Gene Name: DUS2L
• UniProt Synonym Gene Names: DUS2; URLC8; hDUS2
• UniProt Entry Name: DUS2L_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

DUS2L: Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus2 subfamily.

Protein type: RNA-binding; Oxidoreductase; EC 1.3.1.-; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm

Molecular Function: protein binding; FAD binding; protein kinase inhibitor activity; double-stranded RNA binding; tRNA dihydrouridine synthase activity

Biological Process: negative regulation of protein kinase activity