Rabbit anti-Human, Mouse DUS2L Polyclonal Antibody | anti-DUS2L antibody

DUS2L (K450) Polyclonal Antibody

Cat. Num. AAA3002207

• Gene Names: DUS2; SMM1; DUS2L; URLC8
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DUS2L; Polyclonal Antibody; DUS2L (K450) Polyclonal Antibody; tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; Dihydrouridine synthase 2; Up-regulated in lung cancer protein 8; URLC8; tRNA-dihydrouridine synthase 2-like; hDUS2; anti-DUS2L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: URLC8 (K450) polyclonal antibody detects endogenous levels of URLC8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 493

• Applicable Applications for anti-DUS2L antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 420-480 of Human URLC8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of URLC8 (K450) polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:Beas-2B whole cell lysate (30ug) Lane4:SGC7901 whole cell lysate (40ug))

Related Product Information for anti-DUS2L antibody

Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.

NCBI and Uniprot Product Information

• NCBI GI #: 419635745
• NCBI GeneID: 54920
• NCBI Accession #: NP_001258691.1
• NCBI GenBank Nucleotide #: NP_001258691.1
• UniProt Accession #: Q9NX74
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: tRNA-dihydrouridine(20) synthase
• NCBI Official Synonym Full Names: dihydrouridine synthase 2
• NCBI Official Symbol: DUS2
• NCBI Official Synonym Symbols: SMM1; DUS2L; URLC8
• NCBI Protein Information: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Protein Name: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
• UniProt Gene Name: DUS2L
• UniProt Synonym Gene Names: DUS2; URLC8; hDUS2
• UniProt Entry Name: DUS2L_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

DUS2L: Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus2 subfamily.

Protein type: RNA-binding; Oxidoreductase; EC 1.3.1.-; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm

Molecular Function: protein binding; FAD binding; protein kinase inhibitor activity; double-stranded RNA binding; tRNA dihydrouridine synthase activity

Biological Process: negative regulation of protein kinase activity