Western Blot (WB)
(Western blot analysis of CYTB Antibody (Center) in HL-60 cell line lysates (35ug/lane). CYTB (arrow) was detected using the purified Pab.)
Rabbit anti-Human CYTB Polyclonal Antibody | anti-CYTB antibody
CYTB, ID (MT-CYB, COB, CYTB, MTCYB, Cytochrome b, Complex III subunit 3, Complex III subunit III, Cytochrome b-c1 complex subunit 3, Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) (FITC)
Gene Names
MT-CYB; MTCYB; CYTB
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CYTB; Polyclonal Antibody; ID (MT-CYB; COB; MTCYB; Cytochrome b; Complex III subunit 3; Complex III subunit III; Cytochrome b-c1 complex subunit 3; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) (FITC); MT-CYB; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit; anti-CYTB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-CYTB antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CYTB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207~237 amino acids from the Central region of human CYTB.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CYTB Antibody (Center) in HL-60 cell line lysates (35ug/lane). CYTB (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CYTB Antibody (Center) in HL-60 cell line lysates (35ug/lane). CYTB (arrow) was detected using the purified Pab.)
Related Product Information for anti-CYTB antibody
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (By similarity).
Product Categories/Family for anti-CYTB antibody
References
Andrews, R.M., et al. Nat. Genet. 23 (2), 147 (1999). Anderson, S., et al. Nature 290(5806):457-465(1981).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,718 Da
NCBI Official Full Name
cytochrome b (mitochondrion)
NCBI Official Synonym Full Names
mitochondrially encoded cytochrome b
NCBI Official Symbol
MT-CYB
NCBI Official Synonym Symbols
MTCYB; CYTB
NCBI Protein Information
cytochrome b
UniProt Protein Name
Cytochrome b
UniProt Gene Name
MT-CYB
UniProt Synonym Gene Names
COB; CYTB; MTCYB
UniProt Entry Name
CYB_HUMAN
Similar Products
Product Notes
The CYTB mt-cyb (Catalog #AAA6290576) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYTB, ID (MT-CYB, COB, CYTB, MTCYB, Cytochrome b, Complex III subunit 3, Complex III subunit III, Cytochrome b-c1 complex subunit 3, Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYTB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CYTB mt-cyb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYTB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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