Rabbit anti-Mouse, Rat Phospholamban Polyclonal Antibody | anti-PLN antibody

Phospholamban

Cat. Num. AAA628978

• Gene Names: PLN; PLB; CMD1P; CMH18
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Phospholamban; Polyclonal Antibody; Anti -Phospholamban; anti-PLN antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Phospholamban. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

• Applicable Applications for anti-PLN antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of human Phospholamban, identical to the related rat and mouse sequence.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PLN antibody

Phospholamban is a 52aa integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists.Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells.McTiernan et al. (1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles,while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217bp upstream of the transcription start site.

Product Categories/Family for anti-PLN antibody

Antibodies; Abs to Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 190019
• NCBI GeneID: 5350
• UniProt Accession #: P26678; Q5R352
• Molecular Weight: 6,109 Da
• NCBI Official Full Name: phospholamban
• NCBI Official Synonym Full Names: phospholamban
• NCBI Official Symbol: PLN
• NCBI Official Synonym Symbols: PLB; CMD1P; CMH18
• NCBI Protein Information: cardiac phospholamban; OTTHUMP00000017092
• UniProt Protein Name: Cardiac phospholamban
• Protein Family: Cardiac phospholamban
• UniProt Gene Name: PLN
• UniProt Synonym Gene Names: PLB
• UniProt Entry Name: PPLA_HUMAN

NCBI Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq]

Uniprot Description

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: sarcoplasmic reticulum membrane; membrane; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; mitochondrial membrane; vesicle

Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding

Biological Process: cardiac muscle development; negative regulation of calcium ion transport; cytosolic calcium ion homeostasis; negative regulation of heart rate; blood circulation; response to testosterone stimulus; regulation of calcium ion transport; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; regulation of the force of heart contraction; protein homooligomerization

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18