Rabbit anti-Human CLP1 Polyclonal Antibody | anti-CLP1 antibody

CLP1, CT (CLP1, HEAB, Polyribonucleotide 5'-hydroxyl-kinase Clp1, Polynucleotide kinase Clp1, Pre-mRNA cleavage complex II protein Clp1) (AP)

Cat. Num. AAA6286866

• Gene Names: CLP1; HEAB; hClp1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLP1; Polyclonal Antibody; CT (CLP1; HEAB; Polyribonucleotide 5'-hydroxyl-kinase Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1) (AP); Pre-mRNA cleavage complex II protein Clp1; anti-CLP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 425

• Applicable Applications for anti-CLP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CLP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-336 amino acids from the C-terminal region of human CLP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CLP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CLP1 antibody detected the CLP1 protein (arrow).)

Product Categories/Family for anti-CLP1 antibody

Antibodies; Protein Kinases

References

Ramirez, A., et al. RNA 14(9):1737-1745(2008). Weitzer, S., et al. Nature 447(7141):222-226(2007). Paushkin, S.V., et al. Cell 117(3):311-321(2004). Zhou, Z., et al. Nature 419(6903):182-185(2002). de Vries, H., et al. EMBO J. 19(21):5895-5904(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 5803029
• NCBI GeneID: 10978
• NCBI Accession #: NP_006822.1; NP_001136069.1
• NCBI GenBank Nucleotide #: NP_006822.1
• UniProt Accession #: Q92989
• NCBI Official Full Name: polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 1
• NCBI Official Synonym Full Names: cleavage factor polyribonucleotide kinase subunit 1
• NCBI Official Symbol: CLP1
• NCBI Official Synonym Symbols: HEAB; hClp1
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• Protein Family: Tyrosine-protein phosphatase
• UniProt Gene Name: CLP1

NCBI Description

This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.