Rabbit anti-Human CKAP4 Polyclonal Antibody | anti-CKAP4 antibody

CKAP4, CT (CKAP4, Cytoskeleton-associated protein 4, 63-kD cytoskeleton-linking membrane protein) (Biotin)

Cat. Num. AAA6286263

• Gene Names: CKAP4; p63; CLIMP-63; ERGIC-63
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CKAP4; Polyclonal Antibody; CT (CKAP4; Cytoskeleton-associated protein 4; 63-kD cytoskeleton-linking membrane protein) (Biotin); 63-kDa cytoskeleton-linking membrane protein; anti-CKAP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CKAP4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 581-602 amino acids from the C-terminal region of human CKAP4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(CKAP4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CKAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(CKAP4 Antibody (C-term) western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).This demonstrates the CKAP4 antibody detected the CKAP4 protein (arrow).)

Related Product Information for anti-CKAP4 antibody

CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.

Product Categories/Family for anti-CKAP4 antibody

Antibodies; Membrane Proteins

References

Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010). Tomkova, H., et al. Eur J Dermatol 20(3):411-413(2010). Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009). Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009). Bates, S.R., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L658-L669 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 19920317
• NCBI GeneID: 10970
• NCBI Accession #: NP_006816.2
• NCBI GenBank Nucleotide #: NM_006825.3
• UniProt Accession #: Q07065; Q504S5; Q53ES6
• Molecular Weight: 66,022 Da
• NCBI Official Full Name: cytoskeleton-associated protein 4
• NCBI Official Synonym Full Names: cytoskeleton associated protein 4
• NCBI Official Symbol: CKAP4
• NCBI Official Synonym Symbols: p63; CLIMP-63; ERGIC-63
• NCBI Protein Information: cytoskeleton-associated protein 4
• UniProt Protein Name: Cytoskeleton-associated protein 4
• Protein Family: Cytoskeleton-associated protein
• UniProt Gene Name: CKAP4
• UniProt Synonym Gene Names: Climp-63; p63
• UniProt Entry Name: CKAP4_HUMAN

Uniprot Description

CKAP4: High-affinity epithelial cell surface receptor for APF.

Protein type: Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: endoplasmic reticulum membrane; cytoskeleton; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; plasma membrane; lipid particle