Rabbit anti-Human Cdc6 Polyclonal Antibody | anti-Cdc6 antibody

Cdc6, NT (CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, p62(cdc6)) (Azide free) (HRP)

Cat. Num. AAA6283988

• Gene Names: CDC6; CDC18L; HsCDC6; HsCDC18
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Cdc6; Polyclonal Antibody; NT (CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; p62(cdc6)) (Azide free) (HRP); CDC6; p62(cdc6); anti-Cdc6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Cdc6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Cdc6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Cdc6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Cdc6 Antibody (N-term) in Romas cell line lysates (35ug/lane). Cdc6 (arrow) was detected using the purified Pab.)

Related Product Information for anti-Cdc6 antibody

Human Cdc6 is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Product Categories/Family for anti-Cdc6 antibody

Antibodies; Cell Division Cycle (CDC)

References

Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004). Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003). Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003). Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002). Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4502703
• NCBI GeneID: 990
• NCBI Accession #: NP_001245.1
• NCBI GenBank Nucleotide #: NM_001254.3
• UniProt Accession #: Q99741; Q8TB30
• Molecular Weight: 62,720 Da
• NCBI Official Full Name: cell division control protein 6 homolog
• NCBI Official Synonym Full Names: cell division cycle 6
• NCBI Official Symbol: CDC6
• NCBI Official Synonym Symbols: CDC18L; HsCDC6; HsCDC18
• NCBI Protein Information: cell division control protein 6 homolog; p62(cdc6); CDC6-related protein; cdc18-related protein; cell division cycle 6 homolog; CDC6 cell division cycle 6 homolog
• UniProt Protein Name: Cell division control protein 6 homolog
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC6
• UniProt Synonym Gene Names: CDC18L; HsCdc18; HsCDC6
• UniProt Entry Name: CDC6_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC6: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1. Belongs to the CDC6/cdc18 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: nucleoplasm; Golgi apparatus; spindle pole; cytoplasm; spindle midzone; nucleus; cytosol

Molecular Function: protein binding; nucleotide binding; chromatin binding; kinase binding; ATP binding

Biological Process: positive regulation of cytokinesis; mitosis; traversing start control point of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; positive regulation of chromosome segregation; G1/S-specific transcription in mitotic cell cycle; negative regulation of cell proliferation; cell division; negative regulation of DNA replication; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; DNA replication checkpoint; DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 5