Western Blot (WB)
(Western Blot analysis in A549, K562 cell line lysates (35ug/lane) using 033449. This demonstrates that 033449 detected the CD11b protein (arrow).)
Rabbit anti-Human CD11b Polyclonal Antibody | anti-CD11b antibody
CD11b, NT (ITGAM, CD11B, CR3A, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b) (Biotin)
Western Blot (WB)
(Western Blot analysis in A549, K562 cell line lysates (35ug/lane) using 033449. This demonstrates that 033449 detected the CD11b protein (arrow).)
Western Blot (WB)
(Western Blot analysis in A549, K562 cell line lysates (35ug/lane) using 033449. This demonstrates that 033449 detected the CD11b protein (arrow).)
Western Blot (WB)
(Western Blot analysis in human spleen lysate (20ug) using 033449 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 127kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in human spleen lysate (20ug) using 033449 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 127kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in human spleen tissue sections (paraformaldehyde-fixed, paraffin-embedded sections) using 033449. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with 033449 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in human spleen tissue sections (paraformaldehyde-fixed, paraffin-embedded sections) using 033449. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with 033449 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using 033449 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 033449 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using 033449 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 033449 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells (right histogram) using 033449 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells (right histogram) using 033449 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cell surface; plasma membrane; integrin complex; nucleus; external side of plasma membrane
Molecular Function: heparan sulfate proteoglycan binding; heparin binding; protein binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding
Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; activated T cell proliferation; leukocyte migration during inflammatory response; cellular extravasation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; toll-like receptor 4 signaling pathway; leukocyte migration
Disease: Systemic Lupus Erythematosus, Susceptibility To, 6
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Product Notes
The CD11b itgam (Catalog #AAA6282742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD11b, NT (ITGAM, CD11B, CR3A, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD11b itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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