Rabbit anti-Human CARD10 Polyclonal Antibody | anti-CARD10 antibody

CARD10, ID (CARD10, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3) (Biotin)

Cat. Num. AAA6280795

• Gene Names: CARD10; BIMP1; CARMA3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CARD10; Polyclonal Antibody; ID (CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3) (Biotin); CARD-containing MAGUK protein 3; anti-CARD10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CARD10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CARD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-546 amino acids from the Central region of human CARD10.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CARD10 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CARD10 antibody detected the CARD10 protein (arrow).)

Related Product Information for anti-CARD10 antibody

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).

Product Categories/Family for anti-CARD10 antibody

Antibodies; Apoptosis; Caspase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Rehman, A.O., et al. Int J Oral Sci 1(3):105-118(2009). Martin, D., et al. J. Biol. Chem. 284(10):6038-6042(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 51093861
• NCBI GeneID: 29775
• NCBI Accession #: NP_055365.2
• NCBI GenBank Nucleotide #: NM_014550.3
• UniProt Accession #: Q9BWT7; Q14CQ8; Q5TFG6; Q9UGR5; Q9UGR6; Q9Y3H0
• Molecular Weight: 115,931 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 10
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 10
• NCBI Official Symbol: CARD10
• NCBI Official Synonym Symbols: BIMP1; CARMA3
• NCBI Protein Information: caspase recruitment domain-containing protein 10; carma 3; CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; Bcl10 binding protein and activator of NFKB
• UniProt Protein Name: Caspase recruitment domain-containing protein 10
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD10
• UniProt Synonym Gene Names: CARMA3; Carma 3
• UniProt Entry Name: CAR10_HUMAN

NCBI Description

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]

Uniprot Description

CARD10: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: CBM complex; cytoplasm

Molecular Function: protein binding; receptor signaling complex scaffold activity

Biological Process: regulation of apoptosis; activation of NF-kappaB-inducing kinase; protein complex assembly