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Western Blot (WB) (CARD10 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CARD10 antibody detected the CARD10 protein (arrow).)

Rabbit anti-Human CARD10 Polyclonal Antibody | anti-CARD10 antibody

CARD10, ID (CARD10, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3)

Gene Names
CARD10; BIMP1; CARMA3
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
CARD10; Polyclonal Antibody; ID (CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3); Anti -CARD10; anti-CARD10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-CARD10 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
CARD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-546 amino acids from the Central region of human CARD10.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CARD10 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CARD10 antibody detected the CARD10 protein (arrow).)

Western Blot (WB) (CARD10 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CARD10 antibody detected the CARD10 protein (arrow).)
Related Product Information for anti-CARD10 antibody
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).
Product Categories/Family for anti-CARD10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
115,931 Da
NCBI Official Full Name
caspase recruitment domain-containing protein 10
NCBI Official Synonym Full Names
caspase recruitment domain family, member 10
NCBI Official Symbol
CARD10
NCBI Official Synonym Symbols
BIMP1; CARMA3
NCBI Protein Information
caspase recruitment domain-containing protein 10; carma 3; CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; Bcl10 binding protein and activator of NFKB
UniProt Protein Name
Caspase recruitment domain-containing protein 10
UniProt Gene Name
CARD10
UniProt Synonym Gene Names
CARMA3; Carma 3
UniProt Entry Name
CAR10_HUMAN

NCBI Description

The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Activates NF-kappa-B via BCL10 and IKK.

Subunit structure: CARD10 and BCL10 bind to each other by CARD-CARD interaction. They both participate in a complex with MALT1, where MALT1 binds to BCL10

By similarity.

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.

Sequence similarities: Contains 1 CARD domain.

Caution: Supposed to contain a SH3, a PDZ and a guanylate kinase-like domain. But none of these 3 domains are detected by PROSITE, Pfam or SMART.

Research Articles on CARD10

Similar Products

Product Notes

The CARD10 card10 (Catalog #AAA646972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARD10, ID (CARD10, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CARD10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the CARD10 card10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARD10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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