Western Blot (WB)
(CAF-1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).)
Rabbit anti-Human CAF-1 Polyclonal Antibody | anti-CAF-1 antibody
CAF-1, NT (CNOT7, CAF1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1) (APC)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(CAF-1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).)
Western Blot (WB)
(CAF-1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).)
Immunohistochemistry (IHC)
(CAF-1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CAF-1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Apr 2016]
Uniprot Description
CNOT7: Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression. Belongs to the CAF1 family.
Protein type: EC 3.1.13.4; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 8p22-p21.3
Cellular Component: membrane; cytosol; nucleus; CCR4-NOT complex
Molecular Function: signal transducer activity; protein binding; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; exoribonuclease activity; RNA binding; metal ion binding; transcription factor activity
Biological Process: deadenylation-dependent decapping; transcription, DNA-dependent; signal transduction; miRNA-mediated gene silencing; regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; positive regulation of cell proliferation; carbohydrate metabolic process; RNA-mediated gene silencing; positive regulation of transcription from RNA polymerase II promoter; gene expression; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay
Research Articles on CAF-1
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Product Notes
The CAF-1 cnot7 (Catalog #AAA6280475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAF-1, NT (CNOT7, CAF1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAF-1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CAF-1 cnot7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAF-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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