Rabbit anti-Human C11orf74 Polyclonal Antibody | anti-C11orf74 antibody

C11orf74, CT (C11orf74, Uncharacterized protein C11orf74, Protein HEPIS) (APC)

Cat. Num. AAA6279474

• Gene Names: C11orf74; HEPIS
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C11orf74; Polyclonal Antibody; CT (C11orf74; Uncharacterized protein C11orf74; Protein HEPIS) (APC); Protein HEPIS; anti-C11orf74 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-C11orf74 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: C11orf74 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-228 amino acids from the C-terminal region of human C11orf74.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(C11orf74 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the C11orf74 antibody detected the C11orf74 protein (arrow).)

Flow Cytometry (FC/FACS)

(C11orf74 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-C11orf74 antibody

Antibodies; KIAA; orf

References

Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Hong, M., et al. Viral Immunol. 21(2):153-162(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 42476216
• NCBI GeneID: 119710
• NCBI Accession #: NP_620142.2
• NCBI GenBank Nucleotide #: NM_138787.3
• UniProt Accession #: Q86VG3; Q96DD6; D3DR18
• Molecular Weight: 16,847 Da
• NCBI Official Full Name: uncharacterized protein C11orf74 isoform a
• NCBI Official Synonym Full Names: chromosome 11 open reading frame 74
• NCBI Official Symbol: C11orf74
• NCBI Official Synonym Symbols: HEPIS
• NCBI Protein Information: uncharacterized protein C11orf74
• UniProt Protein Name: Uncharacterized protein C11orf74
• UniProt Gene Name: C11orf74
• UniProt Entry Name: CK074_HUMAN

NCBI Description

This gene encodes a protein that was identified as a cellular interacting partner of non-structural protein 10 of the severe acute respiratory syndrome coronavirus (SARS-CoV). The encoded protein may function as a negative regulator of transcription. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

C11orf74: 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11p12