Rabbit ATP5J Polyclonal Antibody | anti-ATP5J antibody

ATP5J (ATP Synthase-coupling Factor 6, Mitochondrial, ATPase Subunit F6, ATP5A, ATPM)

Cat. Num. AAA627635

• Gene Names: ATP5J; F6; CF6; ATP5; ATPM; ATP5A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ATP5J; Polyclonal Antibody; ATP5J (ATP Synthase-coupling Factor 6; Mitochondrial; ATPase Subunit F6; ATP5A; ATPM); Anti -ATP5J (ATP Synthase-coupling Factor 6; anti-ATP5J antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP5J. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

• Applicable Applications for anti-ATP5J antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (paraffin): 1-2ug/ml
• Immunogen: Synthetic peptide mapping at the middle region of human mature ATP5J (CF6), different from the related mouse sequence by 2aa.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Mammary cancer sections Staining ATP-5J in cytoplasm DAB chromogenic reaction)

Western Blot (WB)

(Western blot analysis of Rat Tissue sections (liver) lysis using ATP-5J antibody)

Related Product Information for anti-ATP5J antibody

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72% sequence identity with rat ATP5J.This import signal peptide is rich in basic amino acids, devoid of acidic amino acids, and amphiphilic, which allows it to be water-soluble yet capable of passage through the phospholipid membrane bilayers. Moreover, it is circulating and functions as an endogenous vasoconstrictor by inhibiting cytosolic phospholipase A2.

Product Categories/Family for anti-ATP5J antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 48146017
• NCBI GeneID: 522
• UniProt Accession #: Q6IB54; P18859; Q6NZ59
• Molecular Weight: 12,588 Da
• NCBI Official Full Name: ATP5J
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
• NCBI Official Symbol: ATP5J
• NCBI Official Synonym Symbols: F6; CF6; ATP5; ATPM; ATP5A
• NCBI Protein Information: ATP synthase-coupling factor 6, mitochondrial; ATPase subunit F6; OTTHUMP00000096106; OTTHUMP00000096107; OTTHUMP00000096108; OTTHUMP00000096110; OTTHUMP00000096111; OTTHUMP00000096112; proliferation-inducing protein 36; mitochondrial ATP synthase, subunit F6; mitochondrial ATPase coupling factor 6; mitochondrial ATP synthase, coupling factor 6; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
• UniProt Protein Name: ATP synthase-coupling factor 6, mitochondrial
• Protein Family: ATP synthase-coupling factor
• UniProt Gene Name: ATP5J
• UniProt Entry Name: Q6IB54_HUMAN

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.

Uniprot Description

ATP5J: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. Belongs to the eukaryotic ATPase subunit F6 family.

Protein type: Mitochondrial; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; ATPase activity; transmembrane transporter activity; hydrogen ion transmembrane transporter activity

Biological Process: cellular metabolic process; substantia nigra development; mitochondrial ATP synthesis coupled proton transport