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Western Blot (WB) (Western blot analysis of GARP antibody (Center) in CEM cell line lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse GARP Polyclonal Antibody | anti-LRRC32 antibody

GARP (Glycoprotein A Repetitions Predominant, GARP Protein, Garpin, D11S833E, Leucine-rich Repeat-containing Protein 32, LRRC32)

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified
Purified by ammonium sulfate precipitation.
Synonyms
GARP; Polyclonal Antibody; GARP (Glycoprotein A Repetitions Predominant; GARP Protein; Garpin; D11S833E; Leucine-rich Repeat-containing Protein 32; LRRC32); Anti -GARP (Glycoprotein A Repetitions Predominant; anti-LRRC32 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GARP. Species Crossreactivity: mouse.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-LRRC32 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Immunogen
Synthetic peptide selected from the Center region of human GARP (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GARP antibody (Center) in CEM cell line lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GARP antibody (Center) in CEM cell line lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GARP antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GARP antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). GARP (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded lung carcinoma reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded lung carcinoma reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded cerebellum reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded cerebellum reacted with GARP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-LRRC32 antibody
GARP is a type I membrane protein which contains 20 leucine-rich repeats.
Product Categories/Family for anti-LRRC32 antibody

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
71,979 Da
NCBI Official Full Name
garp
UniProt Protein Name
Leucine-rich repeat-containing protein 32
UniProt Gene Name
LRRC32
UniProt Synonym Gene Names
D11S833E; GARP
UniProt Entry Name
LRC32_HUMAN

Uniprot Description

LRRC32:

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.5-q14

Cellular Component: integral to plasma membrane

Biological Process: negative regulation of activated T cell proliferation; negative regulation of cytokine secretion

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Product Notes

The GARP lrrc32 (Catalog #AAA627580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GARP (Glycoprotein A Repetitions Predominant, GARP Protein, Garpin, D11S833E, Leucine-rich Repeat-containing Protein 32, LRRC32) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GARP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the GARP lrrc32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GARP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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