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Western Blot (WB) (ALPPL2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALPPL2 antibody detected the ALPPL2 protein (arrow).)

Rabbit anti-Human ALPPL2 Polyclonal Antibody | anti-ALPPL2 antibody

ALPPL2, NT (ALPPL2, ALPPL, Alkaline phosphatase, placental-like, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like) (APC)

Gene Names
ALPPL2; ALPG; GCAP; ALPPL
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ALPPL2; Polyclonal Antibody; NT (ALPPL2; ALPPL; Alkaline phosphatase; placental-like; ALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; Placental alkaline phosphatase-like) (APC); Placental alkaline phosphatase-like; anti-ALPPL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ALPPL2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ALPPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the N-terminal region of human ALPPL2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ALPPL2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALPPL2 antibody detected the ALPPL2 protein (arrow).)

Western Blot (WB) (ALPPL2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALPPL2 antibody detected the ALPPL2 protein (arrow).)

Immunohistochemistry (IHC)

(ALPPL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ALPPL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-ALPPL2 antibody
References
Pelttari, K., et al. Arthritis Rheum. 54(10):3254-3266(2006). Nakano, T., et al. Biochem. Biophys. Res. Commun. 341(1):33-38(2006). Hillier, L.W., et al. Nature 434(7034):724-731(2005). Le Du, M.H., et al. J. Biol. Chem. 277(51):49808-49814(2002). Kozlenkov, A., et al. J. Biol. Chem. 277(25):22992-22999(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
251
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,377 Da
NCBI Official Full Name
alkaline phosphatase, placental-like preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental-like 2
NCBI Official Symbol
ALPPL2
NCBI Official Synonym Symbols
ALPG; GCAP; ALPPL
NCBI Protein Information
alkaline phosphatase, placental-like; ALP-1; Nagao isozyme; germ cell alkaline phosphatase; alkaline phosphatase Nagao isozyme; placental-like alkaline phosphatase; testicular and thymus alkaline phosphatase
UniProt Protein Name
Alkaline phosphatase, placental-like
Protein Family
UniProt Gene Name
ALPPL2
UniProt Synonym Gene Names
ALPPL; GCAP; PLAP-like
UniProt Entry Name
PPBN_HUMAN

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]

Uniprot Description

ALPPL2: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, GPI anchor; EC 3.1.3.1; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - folate biosynthesis

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: plasma membrane

Molecular Function: alkaline phosphatase activity; metal ion binding

Biological Process: dephosphorylation

Research Articles on ALPPL2

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Product Notes

The ALPPL2 alppl2 (Catalog #AAA6273071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALPPL2, NT (ALPPL2, ALPPL, Alkaline phosphatase, placental-like, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALPPL2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ALPPL2 alppl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALPPL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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