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Western Blot (WB)
(ALKBH6 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ALKBH6 antibody detected the ALKBH6 protein (arrow).)
Rabbit anti-Human, Mouse ALKBH6 Polyclonal Antibody | anti-ALKBH6 antibody
ALKBH6, ID (ALKBH6, ABH6, Probable alpha-ketoglutarate-dependent dioxygenase ABH6, Alkylated DNA repair protein alkB homolog 6) (AP)
Gene Names
ALKBH6; ABH6
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ALKBH6; Polyclonal Antibody; ID (ALKBH6; ABH6; Probable alpha-ketoglutarate-dependent dioxygenase ABH6; Alkylated DNA repair protein alkB homolog 6) (AP); Alkylated DNA repair protein alkB homolog 6; anti-ALKBH6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ALKBH6 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ALKBH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the Central region of human ALKBH6.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ALKBH6 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ALKBH6 antibody detected the ALKBH6 protein (arrow).)
Western Blot (WB)
(ALKBH6 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ALKBH6 antibody detected the ALKBH6 protein (arrow).)
Western Blot (WB)
(ALKBH6 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the ALKBH6 antibody detected the ALKBH6 protein (arrow).)
Western Blot (WB)
(ALKBH6 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the ALKBH6 antibody detected the ALKBH6 protein (arrow).)
Immunohistochemistry (IHC)
(ALKBH6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALKBH6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ALKBH6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALKBH6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ALKBH6 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ALKBH6 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ALKBH6 antibody
Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).
Product Categories/Family for anti-ALKBH6 antibody
References
Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,483 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 isoform 2
NCBI Official Synonym Full Names
alkB, alkylation repair homolog 6 (E. coli)
NCBI Official Symbol
ALKBH6
NCBI Official Synonym Symbols
ABH6
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 6; alkylation repair homolog 6; alkylated DNA repair protein alkB homolog 6; probable alpha-ketoglutarate-dependent dioxygenase ABH6
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6
Protein Family
UniProt Gene Name
ALKBH6
UniProt Synonym Gene Names
ABH6
Uniprot Description
ALKBH6: Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 19q13.12
Cellular Component: cytoplasm; focal adhesion; nucleoplasm; nucleus
Molecular Function: dioxygenase activity; ferrous iron binding; protein binding
Similar Products
Product Notes
The ALKBH6 alkbh6 (Catalog #AAA6272993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH6, ID (ALKBH6, ABH6, Probable alpha-ketoglutarate-dependent dioxygenase ABH6, Alkylated DNA repair protein alkB homolog 6) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ALKBH6 alkbh6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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