Rabbit anti-Human AIM2 Polyclonal Antibody | anti-AIM2 antibody

AIM2, NT (AIM2, Interferon-inducible protein AIM2, Absent in melanoma 2) (FITC)

Cat. Num. AAA6272435

• Gene Names: AIM2; PYHIN4
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: AIM2; Polyclonal Antibody; NT (AIM2; Interferon-inducible protein AIM2; Absent in melanoma 2) (FITC); Absent in melanoma 2; anti-AIM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AIM2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AIM2 antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 4-32 in the N-terminal region of human AIM2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MCF-7, HL-60, K562, Hela cell line lysates (35ug/lane) usingMBS642376. AIM2 (arrow) was detected usingMBS642376.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MCF-7 cells using MBS642376 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with MCF-7 cell using MBS642376 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-AIM2 antibody

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

Product Categories/Family for anti-AIM2 antibody

Antibodies; Interferon

References

1. Patsos, G., et al. Int. J. Cancer 126(8):1838-1849(2010). 2. Patsos, G., et al. Glycobiology 19(7):726-734(2009). 3. Fernandes-Alnemri, T., et al. Nature 458(7237):509-513(2009). 4. Hornung, V., et al. Nature 458(7237):514-518(2009). 5. Woerner, S.M., et al. Genes Chromosomes Cancer 46(12):1080-1089(2007). 5. Cresswell, K.S., et al. Biochem. Biophys. Res. Commun. 326(2):417-424(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4757734
• NCBI GeneID: 9447
• NCBI Accession #: NP_004824.1
• NCBI GenBank Nucleotide #: NM_004833.1
• UniProt Accession #: O14862; Q5T3V9; Q96FG9; A8K7M7
• Molecular Weight: 38,954 Da
• NCBI Official Full Name: interferon-inducible protein AIM2
• NCBI Official Synonym Full Names: absent in melanoma 2
• NCBI Official Symbol: AIM2
• NCBI Official Synonym Symbols: PYHIN4
• NCBI Protein Information: interferon-inducible protein AIM2
• UniProt Protein Name: Interferon-inducible protein AIM2
• Protein Family: Protein
• UniProt Gene Name: AIM2
• UniProt Entry Name: AIM2_HUMAN

NCBI Description

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]

Uniprot Description

AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; double-stranded DNA binding

Biological Process: positive regulation of protein oligomerization; positive regulation of interleukin-1 beta production; tumor necrosis factor-mediated signaling pathway; apoptosis; inhibition of NF-kappaB transcription factor; innate immune response; immune response; interleukin-1 beta secretion; positive regulation of interleukin-1 beta secretion; inflammatory response; positive regulation of defense response to virus by host; activation of innate immune response; activation of NF-kappaB transcription factor