Typical Testing Data/Standard Curve (for reference only)
Human Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN ELISA Kit | CBR1 elisa kit
Human Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CBR1/CBR/CRN antibody. CBR1/CBR/CRN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CBR1/CBR/CRN Antibody is added and binds to CBR1/CBR/CRN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CBR1/CBR/CRN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CBR1/CBR/CRN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
Uniprot Description
CBR1: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: EC 1.1.1.184; EC 1.1.1.189; EC 1.1.1.197; Lipid Metabolism - arachidonic acid; Oxidoreductase
Chromosomal Location of Human Ortholog: 16 C4|16 54.53 cM
Cellular Component: cytoplasm; extracellular exosome; intracellular membrane-bound organelle; microvillus; nucleus; plasma membrane
Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity
Biological Process: drug metabolic process; vitamin K metabolic process
Research Articles on CBR1
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Product Notes
The Human CBR1 cbr1 (Catalog #AAA1607348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1607348 ELISA Kit recognizes Human CBR1. It is sometimes possible for the material contained within the vial of "Carbonyl reductase [NADPH] 1, CBR1/CBR/CRN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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