Rabbit anti-Mouse Acvr2a Polyclonal Antibody | anti-Acvr2a antibody

Acvr2a, NT (Acvr2a, Acvr2, Activin receptor type-2A, Activin receptor type IIA) (FITC)

Cat. Num. AAA6271423

• Gene Names: Acvr2a; Acvr2; ActrIIa; TactrII
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Acvr2a; Polyclonal Antibody; NT (Acvr2a; Acvr2; Activin receptor type-2A; Activin receptor type IIA) (FITC); Activin receptor type IIA; anti-Acvr2a antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Acvr2a antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Acvr2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human Mouse Acvr2a.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Acvr2a Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Acvr2a antibody detected the Acvr2a protein (arrow).)

Related Product Information for anti-Acvr2a antibody

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.

Product Categories/Family for anti-Acvr2a antibody

Antibodies; Activin

References

Perron, J.C., et al. PLoS ONE 4 (12), E8198 (2009): Morita, S., et al. PLoS ONE 4 (1), E4212 (2009): Kamiya, N., et al. Development 135(22):3801-3811(2008). Xia, Y., et al. J. Biol. Chem. 282(25):18129-18140(2007). Lee, Y.J., et al. Genesis 44(10):487-494(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 160358785
• NCBI GeneID: 11480
• NCBI Accession #: NP_031422.3
• NCBI GenBank Nucleotide #: NM_007396.4
• UniProt Accession #: P27038
• Molecular Weight: 57,890 Da
• NCBI Official Full Name: activin receptor type-2A
• NCBI Official Synonym Full Names: activin receptor IIA
• NCBI Official Symbol: Acvr2a
• NCBI Official Synonym Symbols: Acvr2; ActrIIa; TactrII
• NCBI Protein Information: activin receptor type-2A
• UniProt Protein Name: Activin receptor type-2A
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr2a
• UniProt Synonym Gene Names: Acvr2; ACTR-IIA
• UniProt Entry Name: AVR2A_MOUSE

Uniprot Description

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); EC 2.7.11.30; Kinase, protein; TKL group; STKR family; Type2 subfamily

Cellular Component: cell surface; cytoplasm; integral to membrane; membrane; receptor complex

Molecular Function: activin binding; activin receptor activity; activin receptor activity, type II; ATP binding; coreceptor activity; growth factor binding; kinase activity; metal ion binding; nucleotide binding; PDZ domain binding; protein binding; protein kinase activity; protein self-association; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; transferase activity; transmembrane receptor protein serine/threonine kinase activity

Biological Process: activin receptor signaling pathway; anterior/posterior pattern formation; BMP signaling pathway; determination of left/right symmetry; embryonic skeletal development; gastrulation with mouth forming second; male gonad development; mesoderm development; penile erection; phosphorylation; positive regulation of activin receptor signaling pathway; positive regulation of bone mineralization; positive regulation of erythrocyte differentiation; positive regulation of follicle-stimulating hormone secretion; positive regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of BMP signaling pathway; regulation of nitric-oxide synthase activity; regulation of signal transduction; Sertoli cell proliferation; sperm ejaculation; spermatogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway