Goat anti-Human ALS2CR2 Polyclonal Antibody | anti-STRADB antibody

ALS2CR2 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein, CALS-21, ILP-interacting protein, ILPIP, ILPIPA, MGC102916, PAPK, PRO1038, Pseudokinase ALS2CR2, Serine/threonine-protein kinase ALS2CR2, STE20-related kinase adaptor be

Cat. Num. AAA625904

• Gene Names: STRADB; PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: ALS2CR2; Polyclonal Antibody; ALS2CR2 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein; CALS-21; ILP-interacting protein; ILPIP; ILPIPA; MGC102916; PAPK; PRO1038; Pseudokinase ALS2CR2; Serine/threonine-protein kinase ALS2CR2; STE20-related kinase adaptor be; Anti -ALS2CR2 (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein; anti-STRADB antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALS2CR2. Species sequence homology: canine and bovine.
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-STRADB antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Peptide ELISA Titer: 1:32,000; Western Blot: 1-3ug/ml; ~35kD band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kD according to NP_061041.2 (alpha isoform) and 31.2kD according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293.; Immunohistochemistry: 3-5ug/ml in paraffin embedded Human Heart shows staining of myocardial fibres in transverse section.
• Immunogen: Synthetic peptide corresponding to CDFPDEKDSYWEF, from the C Terminus of human ALS2CR2 (NP_061041.2; AAF71042.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(A1363-10D (1ug/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((A1363-10D(3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Testing Data

(HEK293 overexpressing ILPIP and probed with A1363-10D)

Related Product Information for anti-STRADB antibody

This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene.

Product Categories/Family for anti-STRADB antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 12862480
• NCBI GeneID: 55437
• UniProt Accession #: Q9C0K7; Q5BKY7; Q9P1L0
• Molecular Weight: 47,026 Da
• NCBI Official Full Name: amyotrophic lateral sclerosis 2
• NCBI Official Synonym Full Names: STE20-related kinase adaptor beta
• NCBI Official Symbol: STRADB
• NCBI Official Synonym Symbols: PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
• NCBI Protein Information: STE20-related kinase adapter protein beta; STRAD beta; pseudokinase ALS2CR2; ILP-interacting protein ILPIPA; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
• UniProt Protein Name: STE20-related kinase adapter protein beta
• Protein Family: STE20-related kinase adapter protein
• UniProt Gene Name: STRADB
• UniProt Synonym Gene Names: ALS2CR2; ILPIP; STRAD beta
• UniProt Entry Name: STRAB_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

STLK6: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, STE; STE group; STE20 family; STLK subfamily

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; cell morphogenesis; insulin receptor signaling pathway; JNK cascade; protein export from nucleus; mitotic cell cycle; cell cycle arrest; protein amino acid phosphorylation