Rabbit anti-Rat Bad Polyclonal Antibody

Bad, phosphorylated (Ser136) (Bcl2 Antagonist of Cell Death)

Cat. Num. AAA625648

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Bad; Polyclonal Antibody; phosphorylated (Ser136) (Bcl2 Antagonist of Cell Death); Anti -Bad; anti-Bad antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects transfected levels of Bad only when phosphorylated at Ser136. Does not detect non-phosphorylated Bad or Bad phosphorylated at other sites. Does not detect related family members. Species crossreactivity: mouse. Expected to crossreact with human and rat based on 100% sequence homology.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Bad antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500. Diluted antibody in TBS, 5% BSA, 0.1% Tween-20. Incubate at 4 degree C overnight with gentle shaking.
• Immunogen: Synthetic phosphopeptide corresponding to residues around Ser136 of mouse Bad.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A) or Bad (S112A/S136A), untreated, TPA-treated or forskolin-treated, using B0003-56 (top), MBS625648 (middle) or Bad Antibody (bottom).)

Western Blot (WB)

(Cells transfected with Wild-type Bad, Bad (Ser112A), Bad (S136A) or Bad (S112A/S136A), untreated, TPA-treated or forskolin-treated, using B0003-56 (top), MBS625648 (middle) B0003-47 (bottom).   Western blot analysis of GST-Bad, phosphorylated by CKII or PKA in vitro, using B0003-56 (top), MBS625648 (middle) or Bad Antibody (bottom))

Related Product Information for anti-Bad antibody

Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-xL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA. Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-xL because of its position in the BH3 domain.

Product Categories/Family for anti-Bad antibody

Antibodies; Abs to Apoptosis