Western Blot (WB)
(Western blot analysis of extracts from COLO 201 cells with or without 24 hours’ starvation in serum-free medium (lanes 1 and 2), immunoprecipitates from COLO 201 cells using v-Src antibody treated with or without lambda phosphatase (lanes 3 and 4) and NIH/3T3 cells treated with PDGF (50 ng/ml) for 0, 2 and 5 minutes (lanes 5, 6 and 7, respectively), using MBS625627 (upper) and v-Src antibody (lower).)
Rabbit Src Family Polyclonal Antibody | anti-Src64B antibody
Src Family, phosphorylated (Tyr416)
Gene Names
Src64B; c-src; C-src1; CG7524; D-src; D-Src64B; Dm SRC1; DmelCG7524; DmHD-358; dsrc; dSrc; Dsrc; DSrc; Dsrc64; DSrc64; DSRC64; Dsrc64B; DSrc64B; HD-358; MRE5; src; Src; SRC 64B; src1; Src1; src64; Src64; src64b; src64B
Reactivity
Species Crossreactivity: Mouse, RatSpecies sequence homology: Xenopus 100%
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A chromatography and immunoaffinity chromatography.
Synonyms
Src Family; Polyclonal Antibody; phosphorylated (Tyr416); Anti -Src Family; anti-Src64B antibody
Host
Rabbit
Reactivity
Species Crossreactivity: Mouse, Rat
Species sequence homology: Xenopus 100%
Species sequence homology: Xenopus 100%
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of human Src only when activated by phosphorylation at Tyr416. May crossreact with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. Does not crossreact with Src phosphorylated at Tyr527. May corssreact with phosphorylated RTKs.
Purity/Purification
Affinity Purified
Purified by Protein A chromatography and immunoaffinity chromatography.
Purified by Protein A chromatography and immunoaffinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Concentration
0.021mg/ml (varies by lot)
Applicable Applications for anti-Src64B antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000.
Dilution: Western Blot: 1:1000.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from COLO 201 cells with or without 24 hours’ starvation in serum-free medium (lanes 1 and 2), immunoprecipitates from COLO 201 cells using v-Src antibody treated with or without lambda phosphatase (lanes 3 and 4) and NIH/3T3 cells treated with PDGF (50 ng/ml) for 0, 2 and 5 minutes (lanes 5, 6 and 7, respectively), using MBS625627 (upper) and v-Src antibody (lower).)
Western Blot (WB)
(Western blot analysis of extracts from COLO 201 cells with or without 24 hours’ starvation in serum-free medium (lanes 1 and 2), immunoprecipitates from COLO 201 cells using v-Src antibody treated with or without lambda phosphatase (lanes 3 and 4) and NIH/3T3 cells treated with PDGF (50 ng/ml) for 0, 2 and 5 minutes (lanes 5, 6 and 7, respectively), using MBS625627 (upper) and v-Src antibody (lower).)
Related Product Information for anti-Src64B antibody
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of Tyr527 in the C-terminal tail by Csk renders the enzyme less active (2). Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (4). Lck is essential for T-lymphocyte activation and differentiation (5,6). The activity of Lck is regulated by protein kinases and phosphatases. Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity (7).
Product Categories/Family for anti-Src64B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60 kD
NCBI Official Full Name
Src oncogene at 64B, isoform I
NCBI Official Synonym Full Names
Src oncogene at 64B
NCBI Official Symbol
Src64B
NCBI Official Synonym Symbols
c-src; C-src1; CG7524; D-src; D-Src64B; Dm SRC1; DmelCG7524; DmHD-358; dsrc; dSrc; Dsrc; DSrc; Dsrc64; DSrc64; DSRC64; Dsrc64B; DSrc64B; HD-358; MRE5; src; Src; SRC 64B; src1; Src1; src64; Src64; src64b; src64B
NCBI Protein Information
CG7524 gene product from transcript CG7524-RJ; CG7524-PB; CG7524-PC; CG7524-PD; CG7524-PE; CG7524-PF; CG7524-PG; CG7524-PH; CG7524-PI; CG7524-PJ; Src oncogene at 64B; Src proto oncogene sequence; Src64B-PB; Src64B-PC; Src64B-PD; Src64B-PE; Src64B-PF; Src64B-PG; Src64B-PH; Src64B-PI; Src64B-PJ; mRNA-like ncRNA in embryogenesis 5
Protein Family
Research Articles on Src64B
Similar Products
Product Notes
The Src64B (Catalog #AAA625627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Src Family, phosphorylated (Tyr416) reacts with Species Crossreactivity: Mouse, Rat Species sequence homology: Xenopus 100% and may cross-react with other species as described in the data sheet. AAA Biotech's Src Family can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Src64B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src Family, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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