Western Blot (WB)
(Western blot analysis of anti-VARS Antibody (N-term) in K562 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.)
Rabbit anti-Human Valyl-tRNA Synthetase Polyclonal Antibody | anti-TWN2 antibody
Valyl-tRNA Synthetase, NT (G7A, Protein G7a, Valine-tRNA ligase, ValRS, VARS1, VARS2)
Gene Names
TWN2; T5E21.11; T5E21_11; TWIN 2; TWN2; VALRS; VALYL TRNA SYNTHETASE
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Valyl-tRNA Synthetase; Polyclonal Antibody; NT (G7A; Protein G7a; Valine-tRNA ligase; ValRS; VARS1; VARS2); Anti -Valyl-tRNA Synthetase; anti-TWN2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human VARS.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-TWN2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Immunogen
Synthetic peptide selected from the N-terminal region of human VARS (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-VARS Antibody (N-term) in K562 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-VARS Antibody (N-term) in K562 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-TWN2 antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. This protein belongs to the class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
Product Categories/Family for anti-TWN2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125,926 Da
NCBI Official Full Name
Valyl-tRNA synthetase
NCBI Official Symbol
TWN2
NCBI Official Synonym Symbols
T5E21.11; T5E21_11; TWIN 2; TWN2; VALRS; VALYL TRNA SYNTHETASE
NCBI Protein Information
Valyl-tRNA synthetase
UniProt Protein Name
Valyl-tRNA synthetase
Protein Family
UniProt Gene Name
VALRS
UniProt Entry Name
SYV_ARATH
NCBI Description
Required for proper proliferation of basal cells.
Uniprot Description
Catalytic activity: ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).
Sequence similarities: Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution: The sequence AAB49704.1 differs from that shown. Reason: Frameshift at positions 357 and 361. The sequence AAF63175.1 differs from that shown. Reason: Erroneous gene model prediction.
Similar Products
Product Notes
The TWN2 valrs (Catalog #AAA625234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Valyl-tRNA Synthetase, NT (G7A, Protein G7a, Valine-tRNA ligase, ValRS, VARS1, VARS2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Valyl-tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the TWN2 valrs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Valyl-tRNA Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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