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Western Blot (WB) (Western blot analysis of anti-VARS Antibody (Center) in HL60 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.)

Rabbit VARS Polyclonal Antibody | anti-VARS antibody

VARS Antibody (Center)

Gene Names
VARS; G7A; VARS1; VARS2
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
VARS; Polyclonal Antibody; VARS Antibody (Center); Valine--tRNA ligase; Protein G7a; Valyl-tRNA synthetase; ValRS; G7A; VARS2; anti-VARS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 679-707 amino acids from the Central region of human VARS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
679-707
Sequence Length
297
Applicable Applications for anti-VARS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-VARS Antibody (Center) in HL60 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-VARS Antibody (Center) in HL60 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.)
Related Product Information for anti-VARS antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. This protein belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
References
Jiang,S., FEBS Lett. 579 (27), 6049-6054 (2005)
Bonnefond,L., Biochemistry 44 (12), 4805-4816 (2005)
Xie,T., Genome Res. 13 (12), 2621-2636 (2003)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
140476
NCBI Official Full Name
valine--tRNA ligase
NCBI Official Synonym Full Names
valyl-tRNA synthetase
NCBI Official Symbol
VARS
NCBI Official Synonym Symbols
G7A; VARS1; VARS2
NCBI Protein Information
valine--tRNA ligase
UniProt Protein Name
Valine--tRNA ligase
Protein Family
UniProt Gene Name
VARS
UniProt Synonym Gene Names
G7A; VARS2; ValRS
UniProt Entry Name
SYVC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]

Uniprot Description

VARS: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]

Protein type: EC 6.1.1.9; Translation; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Ligase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: mitochondrion; cytosol

Molecular Function: valine-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; regulation of translational fidelity; gene expression; valyl-tRNA aminoacylation

Research Articles on VARS

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Product Notes

The VARS vars (Catalog #AAA9207475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 679-707. The VARS Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's VARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the VARS vars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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