Rabbit Platelet Derived Growth Factor Receptor beta Polyclonal Antibody

Platelet Derived Growth Factor Receptor beta, phosphorylated (Tyr751) (PDGFRb)

Cat. Num. AAA624791

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Platelet Derived Growth Factor Receptor beta; Polyclonal Antibody; phosphorylated (Tyr751) (PDGFRb); Anti -Platelet Derived Growth Factor Receptor beta; anti-Platelet Derived Growth Factor Receptor beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of PDGF receptor beta only when phosphorylated at Tyr751 (190kD). Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Human, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Platelet Derived Growth Factor Receptor beta antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot and Flow Cytometry.; Dilution: Western Blot: 1:1000; Flow Cytometry: 1:200; Optimal dilution to be determined by researcher
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr751of human PDGF receptor beta (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of cell lysates from various cell lines unstimulated or stimulated with PDGF (40ng/ml for 5 minutes), using Phospho-PDGF Receptor beta (Tyr751).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Phospho-PDGF Receptor beta (Tyr751) staining of untreated (blue) or PDGF treated (green) NIH/3T3 cells compared to a nonspecific negative control antibody (red).)

Related Product Information for anti-Platelet Derived Growth Factor Receptor beta antibody

Platelet-derived growth factor (PDGF) exerts its stimulatory effects on cell growth and motility by binding to two related protein tyrosine kinase receptors, which can associate as three dimer forms (alpha/ alpha, alpha/beta, beta/beta) with variable binding specificity for the PDGF ligands (1,2). The total number of the two receptor subunits and the ratio expressed varies between cell types and appears to account for the variable responsiveness of different cell types to PDGF (3). Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules including Grb2, Src, GAP, PI3 kinase, PLCgamma and Nck. A number of different signaling pathways are thereby initiated leading to cell growth, actin reorganization, migration and differentiation (4). Tyr751 in the kinase-insert region of PDGFR beta is the docking site for PI3 kinase (5). Phosphorylated pentapeptides derived from tyrosine 751 of the PDGFR beta (pTyr751-Val-Pro-Met-Leu) inhibit the association of the C-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFR beta (6).

Product Categories/Family for anti-Platelet Derived Growth Factor Receptor beta antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 1752707
• NCBI Official Full Name: platelet-derived growth factor receptor beta