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Immunocytochemistry (ICC) (Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using hSNF2H (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.)

Mouse anti-Human HSNF2H Monoclonal Antibody | anti-HSNF2H antibody

HSNF2H (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 5, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5, Sucrose Nonfermenting Protein 2 Homolog, SMARCA5, SNF2H, WCRF135) (MaxLight

Gene Names
SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
HSNF2H; Monoclonal Antibody; HSNF2H (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 5; SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5; Sucrose Nonfermenting Protein 2 Homolog; SMARCA5; SNF2H; WCRF135) (MaxLight; anti-HSNF2H antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
13A199
Specificity
Recognizes human HSNF2H.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-HSNF2H antibody
Immunocytochemistry (ICC), Western Blot (WB)
Immunogen
Recombinant corresponding to human HSNF2H fused to GST-tag.
Conjugate
MaxLight405
Preparation and Storage
Store at 4 degree C. Do Not Freeze.

Immunocytochemistry (ICC)

(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using hSNF2H (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.)

Immunocytochemistry (ICC) (Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of HeLa and A431 cells using hSNF2H (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.)

Western Blot (WB)

(Western Blotting analysis: Representative lot data. HeLa cell lysate was probed with hSNF2H (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and visualized using a chemiluminescence detection system. Arrow indicates hSNF2H (~130kD).)

Western Blot (WB) (Western Blotting analysis: Representative lot data. HeLa cell lysate was probed with hSNF2H (0.5ug/ml). Proteins were visualized using a Goat Mouse IgG secondary antibody conjugated to HRP and visualized using a chemiluminescence detection system. Arrow indicates hSNF2H (~130kD).)
Product Categories/Family for anti-HSNF2H antibody
References
1. Gigek, C.O., et al. (2011). Cancer Invest. 29(2):162-166. 2. Precht, P., et al. (2010). Mol Immunol. 47(11- 12):2038-2046. 3. Vargova, J., et al. (2009): Front Biosci (Elite Ed). 1:553-559. 4. Lazzaro, M.A. and Picketts, D.J. (2001). J Neurochem. 77(4):1145-1156.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122kDa
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
NCBI Official Symbol
SMARCA5
NCBI Official Synonym Symbols
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Gene Name
SMARCA5
UniProt Synonym Gene Names
SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
UniProt Entry Name
SMCA5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]

Research Articles on HSNF2H

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Product Notes

The HSNF2H smarca5 (Catalog #AAA6245189) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSNF2H (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 5, SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin A5, Sucrose Nonfermenting Protein 2 Homolog, SMARCA5, SNF2H, WCRF135) (MaxLight reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSNF2H can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the HSNF2H smarca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSNF2H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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