Mouse Protein Phosphatase 2A, Serine, Threonine Monoclonal Antibody | anti-PP2A antibody

Protein Phosphatase 2A, Serine, Threonine (PP2A Ser/Thr) (MaxLight 405)

Cat. Num. AAA6244737

• Gene Names: PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
• Reactivity: Bovine, Human, Mouse, Rabbit, Rat, Xenopus, Yeast
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Protein Phosphatase 2A; Serine; Threonine; Monoclonal Antibody; Threonine (PP2A Ser/Thr) (MaxLight 405); anti-PP2A antibody

• Host: Mouse
• Reactivity: Bovine, Human, Mouse, Rabbit, Rat, Xenopus, Yeast
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 0.T.118
• Specificity: Recognizes human Protein Phosphatase 2A, Serine, Threonine, catalytic subunit, Mr 36kD. Species Crossreactivity: bovine, mouse, rat, rabbit, Xenopus and yeast. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-PP2A antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to aa295-309, CRGEPHVTRRTPDYFL of the 36kD catalytic subunit of human Protein Phosphatase 2A (NM_004156).
• Conjugate: MaxLight405
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Western Blot (WB)

(WB analysis: A431 (lane 1) and 3T3 (lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS609895 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PP2A (36kD).)

Product Categories/Family for anti-PP2A antibody

Antibodies; Enzymes; Phosphatase

References

1. Kremmer, E., et al., Mol. Cell Bio. 17: 1692-1701, 1997. 2. Kawabe, T., et al., Nature 385: 454-458, 1997. 3. Papasozomenos, S.C., and Y. Su, J. Neurochem. 65: 396-406, 1995. 4. Shima, H., et al., Proc. Natl. Acad. Sci. USA 91: 9267-9271. 5. McMahon, K., et al., J. Biochem. 358: 431-436, 2001. 6. Yu, X., Mol. Biol. Cell 12: 185-199, 2001. 7. Thompson, L., et al., J. Biol. Chem. 272: 29693-296697, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 4506017
• NCBI GeneID: 5515
• NCBI Accession #: NP_002706.1
• NCBI GenBank Nucleotide #: NM_004156
• UniProt Accession #: P67775; P05323; P13197
• Molecular Weight: 35,594 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP2CA
• NCBI Official Synonym Symbols: RP-C; PP2Ac; PP2CA; PP2Calpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; replication protein C; protein phosphatase 2A catalytic subunit, alpha isoform; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; serine/threonine pr
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha; RP-C
• UniProt Entry Name: PP2AA_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity

Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process